Nakayama Shizuka, Zhou Jie, Zheng Yue, Szmacinski Henryk, Sintim Herman O
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA.
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA; Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742, USA; Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
Future Sci OA. 2016 Jan 29;2(1):FSO93. doi: 10.4155/fso.15.93. eCollection 2016 Mar.
Cyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine.
METHODS & RESULTS: Atomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA. The stability of c-di-GMP supramolecular polymers is dependent on both the nature of the cation present and the intercalator. C-di-GMP or cyclic diadenylic acid/intercalator complexes are more resistant to cleavage by YybT, a phosphodiesterase, than the uncomplexed nucleotides.
Cleavage of bacterial cyclic dinucleotides could be slowed down via complexation with small molecules and that this could be utilized for diverse applications in nanotechnology and medicine.
环二核苷酸与嵌入剂形成超分子聚集体，这一特性可用于纳米技术和医学领域。
利用原子力显微镜和电泳迁移率变动分析表明，在存在嵌入剂的情况下，环二鸟苷酸（c-di-GMP）形成了G-线。噻唑橙与c-di-GMP结合时的平均荧光寿命长于与DNA G-四链体或双链DNA结合时的平均荧光寿命。c-di-GMP超分子聚合物的稳定性取决于存在的阳离子的性质和嵌入剂。与未复合的核苷酸相比，c-di-GMP或环二腺苷酸/嵌入剂复合物对磷酸二酯酶YybT的切割更具抗性。
通过与小分子复合可减缓细菌环二核苷酸的切割，这可用于纳米技术和医学的多种应用。
