Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia.
Antimicrob Agents Chemother. 2012 Apr;56(4):2166-8. doi: 10.1128/AAC.05796-11. Epub 2012 Jan 17.
Complete sequencing of pJIE137 revealed a backbone closely related to p271A, encoding a novel RepA protein but with a similar organization and up to ∼70% nucleotide identity to IncN plasmids. A region in pJIE137 resembling the IncN CUP regulon is mostly missing from p271A, presumably due to recombination. The class 1 In/Tn and ISEcp1-bla(CTX-M-62) transposition unit in pJIE137 and a putative transposon carrying bla(NDM-1) in p271A are inserted in different locations in the plasmid backbone.
pJIE137 的完整测序结果表明，其骨架与 p271A 密切相关，编码一种新型 RepA 蛋白，但与 IncN 质粒具有相似的组织，且核苷酸同一性高达约 70%。pJIE137 中类似于 IncN CUP 调控子的区域在 p271A 中大部分缺失，推测是由于重组所致。pJIE137 中的类 1 In/Tn 和 ISEcp1-bla(CTX-M-62)转座单元以及 p271A 中携带 bla(NDM-1)的假定转座子均插入在质粒骨架的不同位置。
