基于拉曼光谱的肝细胞癌生物标志物检测

Raman based hepatocellular carcinoma biomarker detection.

作者信息

Santiago-Cordoba M A, Romano P R, MacKay A, Demirel M C

机构信息

Chemistry Department, Pennsylvania State University, University Park, PA 16802, USA.

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:3672-5. doi: 10.1109/IEMBS.2011.6090620.

DOI:10.1109/IEMBS.2011.6090620

PMID:22255136

Abstract

Highly sensitive and selective biomarker detection is required for early detection of hepatocellular carcinoma (HCC). Disease progression has been shown to correlate with specific fucosylation of a validated HCC serum glycoprotein biomarker, alpha-fetoprotein (AFP) Carbohydrate binding proteins, such as lectins, can be used as diagnostic indicators for monitoring glycosylation changes during disease progression in hepatitis B virus (HBV) or hepatitis C virus (HCV) infected patients. We prepared surface-enhanced Raman spectroscopy (SERS) substrates, which provide controllable, well-organized nanoparticles on the surface, for the analysis of a fucose binding lectin AAL. The SERS based assay provides fast (<10 s), and reproducible (<5% variation) detection.

摘要

肝细胞癌（HCC）的早期检测需要高灵敏度和高选择性的生物标志物检测。疾病进展已被证明与一种经过验证的HCC血清糖蛋白生物标志物甲胎蛋白（AFP）的特定岩藻糖基化相关。碳水化合物结合蛋白，如凝集素，可作为诊断指标，用于监测乙型肝炎病毒（HBV）或丙型肝炎病毒（HCV）感染患者疾病进展过程中的糖基化变化。我们制备了表面增强拉曼光谱（SERS）基底，其在表面提供可控、有序的纳米颗粒，用于分析岩藻糖结合凝集素AAL。基于SERS的检测方法提供了快速（<10秒）且可重复（变异<5%）的检测。