Key Laboratory of Functional Materials Physics and Chemistry, Ministry of Education, College of Physics , Jilin Normal University , Changchun 130103 , P.R. China.
School of Material and Environmental Engineering , Hangzhou Dianzi University , Hangzhou 310018 , P.R. China.
ACS Appl Mater Interfaces. 2019 Nov 27;11(47):44617-44623. doi: 10.1021/acsami.9b16288. Epub 2019 Nov 15.
Overexpression of the agglutinin-reactive fraction of alpha-fetoprotein (AFP-L3) is an essential biomarker for early diagnosis of hepatocellular carcinoma (HCC). In this study, we designed a new surface-enhanced Raman spectroscopy active chip for the detection of AFP with high sensitivity and excellent repeatability. This chip was composed of a honeycomb gold nanostructure array with strong electromagnetic field coupling due to the special cavity geometric characteristics of the honeycomb structure. The honeycomb structure exhibited extraordinary performance for the specific detection of AFP in the range of 0.003-3 ng/mL and also determined the proportion of AFP-L3 with a high degree of accuracy, which has shown great potential for application in the clinical diagnosis of HCC.
甲胎蛋白（AFP）凝集素反应片段的过表达是肝细胞癌（HCC）早期诊断的重要生物标志物。在这项研究中，我们设计了一种新的表面增强拉曼光谱活性芯片，用于高灵敏度和出色重复性的 AFP 检测。该芯片由具有强电磁场耦合的蜂窝状金纳米结构阵列组成，这是由于蜂窝结构的特殊腔几何特性所致。在 0.003-3ng/mL 的范围内，蜂窝结构对 AFP 的特异性检测表现出非凡的性能，并且还以高精度确定了 AFP-L3 的比例，这在 HCC 的临床诊断中具有很大的应用潜力。
