Wang Hong, Liu Yina, Zeng Zuping, He Wei
Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2525-9.
To establish the chromatographic fingerprints for the anti-tumor flavonoids of Caulis spatholobi (SSCE). It could used to reflect the chemical information in this part comprehensively, and identify the chemical consitituents preliminarily.
The HPLC-DAD analysis method was performed on the column Kromasil 100-5PHENYL (4.6 mm x 250 mm, 5 microm). The mobile phase was water (0.5% acetic acid)- methanol in gradient elution and the detection wavelength was 254 nm.
The chromatographic fingerprint of SSCE was established, which showed 16 characteristic peaks from 10 batches of medicinal materials. Among them, the peaks 1, 3, 4, 5, 8, 9, 10, 12, 13, and 16 were identified 3,4-dihodroxybenzoic acid, 4-Hydroxybenzoic Acid, epicatechin, puerarin, daidzein, liquiritigenin, calycosin, genistein, formononetin, and prunetin, respectively.
The method is convenient, reproducibility and stability. It can used for quality control of the anti-tumor flavonoids of C. spatholobi (SSCE).
建立鸡血藤抗肿瘤黄酮部位（SSCE）的色谱指纹图谱，以全面反映该部位的化学信息，并初步鉴定其化学成分。
采用HPLC-DAD分析方法，色谱柱为Kromasil 100-5PHENYL（4.6 mm×250 mm，5μm），流动相为水（0.5%乙酸）-甲醇梯度洗脱，检测波长为254 nm。
建立了SSCE的色谱指纹图谱，10批药材共显示16个特征峰。其中，峰1、3、4、5、8、9、10、12、13、16分别鉴定为3,4-二羟基苯甲酸、4-羟基苯甲酸、表儿茶素、葛根素、大豆苷元、甘草素、毛蕊异黄酮、染料木素、芒柄花素和樱黄素。
该方法简便、重现性好、稳定性高，可用于鸡血藤抗肿瘤黄酮部位（SSCE）的质量控制。
