Rodríguez-Martínez José M, Conejo M Carmen, Díaz de Alba Paula, López-Cerero Lorena, Fernandez-Echauri Pedro, Pascual Alvaro
Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Sevilla, España.
Enferm Infecc Microbiol Clin. 2012 May;30(5):246-8. doi: 10.1016/j.eimc.2011.09.020. Epub 2012 Jan 16.
Combined resistance to quinolones and β-lactams is common in Enterobacteriaceae. The appearance in enterobacteria coding for metallo-β-lactamases and determinants of plasmid-mediated quinolone resistance are an emerging problem in our country.
The susceptibility was determined by E-test. The resistance genes were detected by PCR and the corresponding plasmids were characterised.
This study describes 2 strains (1 Klebsiella oxytoca, 1 Klebsiella pneumoniae) carrying the genes qnrS2 and blaVIM-1 in a transferable plasmid of 70-Kb isolated in surveillance cultures at the University Hospital Virgen Macarena in Seville.
This is the first combination of qnrS2 and bla(VIM-1) on the same non-typeable plasmid isolated in our centre.
肠杆菌科细菌对喹诺酮类和β-内酰胺类药物的联合耐药很常见。编码金属β-内酰胺酶的肠杆菌以及质粒介导的喹诺酮耐药决定子的出现是我国一个新出现的问题。
采用E-test法测定药敏。通过PCR检测耐药基因并对相应质粒进行特征分析。
本研究描述了在塞维利亚圣玛利亚大学医院监测培养物中分离出的2株菌株（1株产酸克雷伯菌、1株肺炎克雷伯菌），它们在一个70-Kb的可转移质粒中携带qnrS2和blaVIM-1基因。
这是在我们中心分离出的同一不可分型质粒上首次出现qnrS2和bla(VIM-1)的组合。
