Laser Zentrum Hannover e.V., Hannover, Germany.
Biofabrication. 2012 Mar;4(1):015001. doi: 10.1088/1758-5082/4/1/015001. Epub 2012 Jan 18.
Fabrication of three-dimensional (3D) fibrin scaffolds with tightly controllable pore sizes and interconnections has been investigated. The scaffolds were produced using a combination of two-photon polymerization (2PP) and micromolding techniques. Master structures were fabricated by 2PP and regenerated in fibrin by a two-step microreplication procedure. Scanning electron and optical microscopy observations showed that the fibrin scaffolds exhibited a highly porous and interconnected structure. Seeding of endothelial cells in fibrin scaffolds resulted in their directed lining and spreading within network of microreplicated pores, whereas encapsulation of endothelial cells in fibrin gel blocks led to their chaotic and irregular distribution within constructs. These results demonstrate that the 2PP-micromolding technique is suitable for fabrication of complex 3D structures from natural proteins for tissue engineering applications.
已经研究了具有紧密可控孔径和连通性的三维（3D）纤维蛋白支架的制造。这些支架是通过双光子聚合（2PP）和微成型技术的组合制造的。母结构通过 2PP 制造，并通过两步微复制程序在纤维蛋白中再生。扫描电子显微镜和光学显微镜观察表明，纤维蛋白支架具有高度多孔和相互连通的结构。内皮细胞在纤维蛋白支架中的接种导致它们在微复制孔的网络中定向排列和扩散，而内皮细胞在纤维蛋白凝胶块中的包封导致它们在构建物内杂乱无章和不规则的分布。这些结果表明，2PP-微成型技术适合用于制造组织工程应用中天然蛋白质的复杂 3D 结构。
