Institute of Integrative and Comparative Biology University of Leeds, Leeds LS2 9JT, UK.
Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x. Epub 2012 Jan 20.
Comparative ecological and behavioural studies of the widespread and diverse Vespertilionidae, which comprise almost 400 of the 1100 bat species, have been limited by the availability of markers. The potential of new methods for developing conserved microsatellite markers that possess enhanced cross-species utility has recently been illustrated in studies of birds. We have applied these methods to develop enhanced microsatellite markers for vespertilionid bats, in particular for the genus Myotis (103 species). We compared published bat microsatellites with their homologues in the genome sequence of the little brown bat, Myotis lucifugus, to create consensus sequences that were used to design candidate primer sets. Primer sets were then tested for amplification and polymorphism in 22 species of bat from nine of the largest families (including 11 Vespertilionidae). Of 46 loci tested, 33 were polymorphic, on average, in each of seven Myotis species tested, 20 in each of four species in other vespertilionid genera, and two in 11 nonvespertilionid species.
对广泛多样的蝙蝠科（包含了 1100 种蝙蝠中的近 400 种）进行比较生态学和行为学研究受到标记物可用性的限制。在鸟类研究中，最近已经展示了新方法开发具有增强跨物种通用性的保守微卫星标记物的潜力。我们已经应用这些方法为蝙蝠科，特别是蝙蝠属（103 种）开发了增强型微卫星标记物。我们将已发表的蝙蝠微卫星与小褐蝠（Myotis lucifugus）的基因组序列中的同源物进行比较，创建了用于设计候选引物对的共识序列。然后，在来自 9 个最大科的 22 种蝙蝠中测试了引物对的扩增和多态性（包括 11 种蝙蝠科）。在测试的 46 个基因座中，在测试的 7 种 Myotis 物种中的每个物种中平均有 33 个多态性，在其他蝙蝠属的 4 种物种中的每个物种中有 20 个，在 11 种非蝙蝠科物种中有 2 个。
