Department of Plastic Surgery, Kyorin University School of Medicine, Mitaka-shi, Tokyo, Japan.
Connect Tissue Res. 2012;53(5):349-54. doi: 10.3109/03008207.2012.657309. Epub 2012 Jul 24.
Clinically, wounds on the face tend to heal with less scarring than those on the trunk, but the causes of this difference have not been clarified. Fibroblasts obtained from different parts of the body are known to show different properties. To investigate whether the characteristic properties of facial and trunk wound healing are caused by differences in local fibroblasts, we comparatively analyzed the functional properties of superficial and deep dermal fibroblasts obtained from the facial and trunk skin of seven individuals, with an emphasis on tendency for fibrosis. Proliferation kinetics and mRNA and protein expression of 11 fibrosis-associated factors were investigated. The proliferation kinetics of facial and trunk fibroblasts were identical, but the expression and production levels of profibrotic factors, such as extracellular matrix, transforming growth factor-β1, and connective tissue growth factor mRNA, were lower in facial fibroblasts when compared with trunk fibroblasts, while the expression of antifibrotic factors, such as collagenase, basic fibroblast growth factor, and hepatocyte growth factor, showed no clear trends. The differences in functional properties of facial and trunk dermal fibroblasts were consistent with the clinical tendencies of healing of facial and trunk wounds. Thus, the differences between facial and trunk scarring are at least partly related to the intrinsic nature of the local dermal fibroblasts.
临床上，面部伤口的愈合往往比躯干伤口的愈合留下的疤痕更少，但造成这种差异的原因尚不清楚。众所周知，从身体不同部位获得的成纤维细胞表现出不同的特性。为了研究面部和躯干伤口愈合的特征性是否是由局部成纤维细胞的差异引起的，我们比较分析了 7 名个体的面部和躯干皮肤浅层和深层真皮成纤维细胞的功能特性，重点研究纤维化倾向。研究了 11 种纤维化相关因子的增殖动力学以及 mRNA 和蛋白表达。面部和躯干成纤维细胞的增殖动力学相同，但与躯干成纤维细胞相比，面部成纤维细胞中细胞外基质、转化生长因子-β1 和结缔组织生长因子 mRNA 等促纤维化因子的表达和产生水平较低，而胶原酶、碱性成纤维细胞生长因子和肝细胞生长因子等抗纤维化因子的表达则没有明显的趋势。面部和躯干真皮成纤维细胞功能特性的差异与面部和躯干伤口愈合的临床趋势一致。因此，面部和躯干瘢痕形成的差异至少部分与局部真皮成纤维细胞的固有性质有关。
