School of Energy, Environmental, Biological and Medical Engineering, University of Cincinnati, 701 Engineering Research Center, Cincinnati, Ohio 45221-0071, United States.
Environ Sci Technol. 2012 Feb 21;46(4):2352-9. doi: 10.1021/es2037993. Epub 2012 Feb 7.
The potential ecological impacts of aerobic biodegradation of vegetable oils on contaminated water columns was investigated in the laboratory at different oil loadings (100, 333, and 1,000 gal acre(-1)) and mixing regimes (fully, moderately, and nonmixed microcosms). The impacts were estimated by use of the Microtox assay and dissolved oxygen concentration measurements. The results of the Microtox assay showed no major toxicity at the 100 gal acre(-1) loading. Furthermore, oxygen was not completely depleted from the water column at this oil coverage. At higher oil loadings, oxygen was fully depleted from the mixed and nonmixed water columns. A transient toxicity in the aqueous phase was observed in the case of the moderately mixed microcosms at 333 gal acre(-1) and was maintained at moderate levels (EC(50) ∼ 30%) in the nonmixed microcosms. A substantial increase in toxicity (EC(50) ∼ 10%) was observed in both mixing conditions when the initial oil loading was increased to 1,000 gal acre(-1). At all oil loadings, significant toxicity (EC(50) < 2%) was found in the solid phase due to the strong partition of lipids to the biomass. Long and medium chains fatty acids associated with the measured toxicity were detected in both liquid and solid phases.
研究了在不同油负荷（100、333 和 1000 加仑/英亩）和混合状态（完全、适度和非混合微宇宙）下，好氧生物降解植物油对受污染水柱的潜在生态影响。使用 Microtox 测定法和溶解氧浓度测量来估计影响。Microtox 测定结果表明，在 100 加仑/英亩的负荷下，没有明显的毒性。此外，在这种油覆盖下，水中的氧气并没有完全耗尽。在更高的油负荷下，混合和非混合水柱中的氧气完全耗尽。在 333 加仑/英亩的适度混合微宇宙中，观察到水相中的瞬态毒性，并在非混合微宇宙中保持在中等水平（EC（50）∼30%）。当初始油负荷增加到 1000 加仑/英亩时，在两种混合条件下都观察到毒性显著增加（EC（50）∼10%）。在所有油负荷下，由于脂质强烈分配到生物量中，在固相中发现了显著的毒性（EC（50）<2%）。在液相和固相中都检测到与测量毒性相关的长链和中链脂肪酸。
