Biomedicinal Information Research Center (BIRC), Japan Biological Informatics Consortium (JBIC), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
J Nat Prod. 2012 Feb 24;75(2):280-4. doi: 10.1021/np2008279. Epub 2012 Jan 20.
The search for metabolites of Kibdelosporangium sp. AK-AA56 resulted in the discovery of novel N-phenylacetylated peptides, JBIR-78 (1) and JBIR-95 (2). Compounds 1 and 2 were established to be N-phenylacetylated heptapeptides by extensive NMR and HRESIMS analyses. The absolute configuration of the standard amino acids including a cysteic acid moiety was determined using Marfey's method on the acid hydrolysates of 1 and 2. The relative and absolute configurations of a nonstandard amino acid, β-hydroxyleucine, were elucidated using the J-based and modified Mosher's methods, respectively. In an antimicrobial test, 1 showed antibacterial activity against Micrococcus luteus.
从 Kibdelosporangium sp. AK-AA56 的代谢产物中发现了新型的 N-苯乙酰化肽，JBIR-78（1）和 JBIR-95（2）。通过广泛的 NMR 和 HRESIMS 分析，确定化合物 1 和 2 为 N-苯乙酰化七肽。使用 Marfey 法对 1 和 2 的酸水解产物进行分析，确定了包括半胱氨酸部分的标准氨基酸的绝对构型。使用 J 基于和改进的 Mosher 方法，分别阐明了非标准氨基酸β-羟基亮氨酸的相对和绝对构型。在抗菌试验中，1 对微球菌显示出抗菌活性。
