来自稀有放线菌 Actinomadura sp. KC 191 的抗菌 Norditerpenoid 放线杜醇

Actinomadurol, an Antibacterial Norditerpenoid from a Rare Actinomycete, Actinomadura sp. KC 191.

机构信息

School of Biology, Newcastle University , Newcastle upon Tyne, NE1 7RU, United Kingdom.

出版信息

J Nat Prod. 2016 Jul 22;79(7):1886-90. doi: 10.1021/acs.jnatprod.6b00268. Epub 2016 Jul 1.

DOI:10.1021/acs.jnatprod.6b00268

Abstract

A new secondary metabolite, actinomadurol (1), was isolated along with the known compound JBIR-65 (2) from a rare actinomycete, Actinomadura strain KC 191. The structure of 1 was established as a rare member of the bacterial C-19 norditerpenoid class by NMR data and ECD calculations. The absolute configuration of 2, which was previously reported without stereochemical analysis, was determined by using the modified Mosher's method and ECD calculations. Actinomadurol (1) exhibited potent antibacterial activity against pathogenic strains, such as Staphylococcus aureus, Kocuria rhizophila, and Proteus hauseri (MIC = 0.39-0.78 μg/mL), whereas JBIR-65 (2) showed no antibacterial activity.

摘要

从一种罕见的放线菌 Actinomadura 菌株 KC 191 中分离得到一种新的次生代谢产物 actinomadurol（1）和已知化合物 JBIR-65（2）。通过 NMR 数据和 ECD 计算，确定 1 的结构为细菌 C-19 降二萜类的罕见成员。之前报道的 2 没有进行立体化学分析，通过使用改进的 Mosher 法和 ECD 计算确定其绝对构型。Actinomadurol（1）对金黄色葡萄球菌、根瘤菌和变形杆菌等致病菌表现出强烈的抗菌活性（MIC = 0.39-0.78 μg/mL），而 JBIR-65（2）则没有抗菌活性。