Marques e Silva Silvana, Ferreira Vânia Maria Moraes, Carneiro Fabiana Pirani, Feres Omar, de Oliveira Paulo Gonçalves, de Sousa João Batista
Faculdade de Medicina, Universidade de Brasília-UNB, DF, Brazil.
Rev Col Bras Cir. 2011 Nov-Dec;38(6):429-34.
To evaluate the effects of bromopride on the formation of adhesions and anastomotic healing in the left colon of rats.
We divided 40 rats into two groups of 20 animals, administration of bromopride (study group-E) or saline (control group-C). Each group was divided into subgroups containing 10 animals each for euthanasia in the third (C3 and E3) or the seventh (E7 and C7) postoperative days. The rats were submitted to section of the left colon and end-to-end anastomosis. On the day of reoperation, we evaluated the total amount of adhesions and removed a colonic segment containing the anastomosis for histopathological analysis, assessment of rupture strength and hydroxyproline concentration.
There was no difference between groups in relation to clinical outcome. Two animals in the study group had blocked anastomotic leakage. The animals that received bromopride had the number of intracavitary adhesions and adhesions to the anastomosis similar to the control group. The anastomoses from the group E3 animals showed lower resistance to rupture the one from the C3 group (p = 0.04). This effect did not occur on the seventh postoperative day (p = 0.37). There was no significant difference between groups in relation to histopathology and hydroxyproline concentration in the anastomoses.
The use of bromopride was associated with decreased tensile strength of left colon anastomosis in rats in the third postoperative day.
评估溴必利对大鼠左半结肠粘连形成及吻合口愈合的影响。
将40只大鼠分为两组，每组20只，分别给予溴必利（研究组-E）或生理盐水（对照组-C）。每组再分为两个亚组，每组10只，分别在术后第3天（C3和E3）或第7天（E7和C7）实施安乐死。对大鼠进行左半结肠切断及端端吻合术。再次手术当天，评估粘连总量，并切除包含吻合口的结肠段进行组织病理学分析、破裂强度评估及羟脯氨酸浓度测定。
两组临床结局无差异。研究组有2只动物发生吻合口漏阻塞。接受溴必利的动物腔内粘连数量及吻合口粘连情况与对照组相似。E3组动物的吻合口破裂阻力低于C3组（p = 0.04）。术后第7天未出现此效应（p = 0.37）。两组在吻合口组织病理学及羟脯氨酸浓度方面无显著差异。
术后第3天，使用溴必利会使大鼠左半结肠吻合口的抗张强度降低。
