Silva Silvana Marques e, Carneiro Fabiana Pirani, Oliveira Paulo Gonçalves de, Morais Pedro Henrique Alves de, Silva Naiara Galvão da, Sousa João Batista de
Medical School, UnB, Brasilia, DF, Brazil.
Acta Cir Bras. 2012 Jun;27(6):370-5. doi: 10.1590/s0102-86502012000600003.
To evaluate the effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis.
Forty rats were divided into two groups to receive either bromopride (experimental group- E) or saline (control group- C). Each group was divided into subgroups of ten animals each to be euthanized on third (E3 and C3) or seventh day (E7 and C7) after surgery. Sepsis was induced by cecal ligation and puncture. The rats underwent segmental left colon resection and end-to-end anastomosis. Adhesion formation, tensile strength and hydroxyproline concentration were assessed. Histomorphometry of collagen and histopathological analysis were also performed.
On postoperative third day, anastomoses in bromopride-treated animals showed lower tensile strength (p=0.02) and greater reduction in hydroxyproline concentration (p=0.04) than in control animals. There was no statistical difference in these parameters on seventh day, and the remaining parameters were similar across subgroups. Collagen content was also similar across subgroups.
In the presence of abdominal sepsis, the administration of bromopride was associated with decreased tensile strength and hydroxyproline concentration in left colonic anastomoses in rats three days after surgery.
评估溴必利对诱发腹部脓毒症大鼠左结肠吻合口愈合的影响。
40只大鼠分为两组，分别接受溴必利（实验组-E）或生理盐水（对照组-C）。每组再分为各含10只动物的亚组，分别在术后第3天（E3和C3）或第7天（E7和C7）实施安乐死。通过盲肠结扎和穿刺诱发脓毒症。大鼠接受左半结肠节段切除及端端吻合术。评估粘连形成、抗张强度和羟脯氨酸浓度。还进行了胶原组织形态计量学和组织病理学分析。
术后第3天，与对照动物相比，接受溴必利治疗的动物吻合口抗张强度较低（p=0.02），羟脯氨酸浓度降低幅度更大（p=0.04）。第7天这些参数无统计学差异，其余参数在各亚组中相似。各亚组的胶原含量也相似。
在存在腹部脓毒症的情况下，术后三天给予溴必利与大鼠左结肠吻合口抗张强度降低及羟脯氨酸浓度降低有关。
