Bosmans Joanna W A M, Moossdorff Martine, Al-Taher Mahdi, van Beek Lotte, Derikx Joep P M, Bouvy Nicole D
Department of General Surgery, Maastricht University Medical Centre, P.O. Box 5800, 6202, AZ, Maastricht, The Netherlands.
NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.
Int J Colorectal Dis. 2016 May;31(5):1021-1030. doi: 10.1007/s00384-016-2550-5. Epub 2016 Mar 10.
This project aimed to reach consensus on the most appropriate animal models and outcome measures in research on anastomoses in the lower gastrointestinal tract (GIT). The physiology of anastomotic healing remains an important research topic in gastrointestinal surgery. Recent results from experimental studies are limited with regard to comparability and clinical translation.
PubMed and EMBASE were searched for experimental studies investigating anastomotic healing in the lower GIT published between January 1, 2000 and December 31, 2014 to assess currently used models. All corresponding authors were invited for a Delphi-based analysis that consisted of two online survey rounds followed by a final online recommendation survey to reach consensus on the discussed topics.
Two hundred seventy-seven original articles were retrieved and 167 articles were included in the systematic review. Mice, rats, rabbits, pigs, and dogs are currently being used as animal models, with a large variety in surgical techniques and outcome measures. Forty-four corresponding authors participated in the Delphi analysis. In the first two rounds, 39/44 and 35/39 participants completed the survey. In the final meeting, 35 experts reached consensus on 76/122 items in six categories. Mouse, rat, and pig are considered appropriate animal models; rabbit and dog should be abandoned in research regarding bowel anastomoses. ARRIVE guidelines should be followed more strictly.
Consensus was reached on several recommendations for the use of animal models and outcome measurements in research on anastomoses of the lower GIT. Future research should take these suggestions into account to facilitate comparison and clinical translation of results.
本项目旨在就下消化道（GIT）吻合术研究中最合适的动物模型和结局指标达成共识。吻合口愈合的生理学仍是胃肠外科的一个重要研究课题。近期实验研究的结果在可比性和临床转化方面存在局限性。
检索PubMed和EMBASE数据库，查找2000年1月1日至2014年12月31日期间发表的关于下消化道吻合口愈合的实验研究，以评估当前使用的模型。邀请所有通讯作者参与基于德尔菲法的分析，该分析包括两轮在线调查，随后进行一轮最终在线推荐调查，以就讨论的主题达成共识。
检索到277篇原始文章，167篇文章纳入系统评价。目前小鼠、大鼠、兔子、猪和狗被用作动物模型，手术技术和结局指标差异很大。44位通讯作者参与了德尔菲分析。在前两轮中，分别有39/44和35/39名参与者完成了调查。在最终会议上，35位专家就六个类别的122项中的76项达成了共识。小鼠、大鼠和猪被认为是合适的动物模型；在肠道吻合术研究中应摒弃兔子和狗。应更严格地遵循ARRIVE指南。
就下消化道吻合术研究中动物模型的使用和结局测量的若干建议达成了共识。未来的研究应考虑这些建议，以促进结果的比较和临床转化。
