心脏兰尼碱受体/钙释放通道cDNA的一级结构与功能表达

Primary structure and functional expression from cDNA of the cardiac ryanodine receptor/calcium release channel.

作者信息

Nakai J, Imagawa T, Hakamat Y, Shigekawa M, Takeshima H, Numa S

机构信息

Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.

出版信息

FEBS Lett. 1990 Oct 1;271(1-2):169-77. doi: 10.1016/0014-5793(90)80399-4.

DOI:10.1016/0014-5793(90)80399-4

PMID:2226801

Abstract

The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca2(+)-dependent Cl- current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA.

摘要

通过对兔心肌肌浆网中兰尼碱受体（ryanodine receptor）的cDNA进行克隆和测序，已推断出其由4968个（插入一个氨基酸后为4976个）氨基酸组成的序列。该蛋白质在氨基酸序列上具有同源性，并且与骨骼肌兰尼碱受体具有共同的特征性结构特点。注射了源自心肌兰尼碱受体cDNA的mRNA的非洲爪蟾卵母细胞，在咖啡因刺激下表现出Ca2(+)-依赖性Cl-电流，这表明形成了功能性钙释放通道。用针对心肌兰尼碱受体mRNA的特异性探针进行RNA印迹杂交分析表明，胃和脑中含有一种可杂交的RNA物种，其大小与心肌mRNA相似。这一结果，结合部分cDNA序列的克隆和分析，提示脑中含有心肌型兰尼碱受体mRNA。