Takeshima H, Nishimura S, Nishi M, Ikeda M, Sugimoto T
International Institute for Advanced Studies, Kyoto, Japan.
FEBS Lett. 1993 May 10;322(2):105-10. doi: 10.1016/0014-5793(93)81547-d.
We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size of approximately 2,400 nucleotides hybridizable with the 3'-terminal region of the skeletal muscle ryanodine receptor cDNA. cDNA cloning and genome analysis indicated that two transcripts differing in their start sites are produced from the skeletal muscle ryanodine receptor gene in a tissue-specific fashion, and that the mRNA in brain may code the carboxyl-terminal region of the ryanodine receptor molecule. cDNA expression experiments suggested that the ATG triplet encoding Met4382 of the skeletal muscle ryanodine receptor can function as a translation initiation codon, and that the expressed protein composed of the carboxy terminal 656 amino acid residues of the receptor is located on the endoplasmic reticulum membrane.
我们之前已经表明，约16,000个核苷酸的骨骼肌兰尼碱受体mRNA编码构成骨骼肌钙释放通道的5,037个氨基酸残基。在本研究中，RNA印迹杂交分析表明，大脑中含有一种估计大小约为2,400个核苷酸的RNA，可与骨骼肌兰尼碱受体cDNA的3'末端区域杂交。cDNA克隆和基因组分析表明，骨骼肌兰尼碱受体基因以组织特异性方式产生两种起始位点不同的转录本，并且大脑中的mRNA可能编码兰尼碱受体分子的羧基末端区域。cDNA表达实验表明，编码骨骼肌兰尼碱受体Met4382的ATG三联体可作为翻译起始密码子，并且由受体羧基末端656个氨基酸残基组成的表达蛋白位于内质网膜上。
