Grupo de Investigación en Polifenoles (GIP), Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
J Agric Food Chem. 2012 Feb 15;60(6):1373-9. doi: 10.1021/jf203836a. Epub 2012 Feb 6.
Detection and individual quantification of oak wood ellagitannins in oak barrel aged red wine samples are difficult mainly due to their low levels and the similarity between their structures. In this work, a quantification method using mass spectrometry has been developed and validated to quantify wine ellagitannins after sample fractionation with a previously reported method. The use of an internal standard is a requirement to correct mass signal variability. (-)-Gallocatechin, among the different tested compounds, was the only one that proved to be a suitable internal standard making possible the accurate and individual quantification of the main oak wood ellagitannins. The developed methodology has been used to detect and quantify these ellagitannins in different Spanish commercial wines, proving its usefulness.
检测和定量分析橡木桶陈酿红酒样品中的橡木单宁非常困难，主要是因为它们的含量低，而且结构相似。在这项工作中，开发并验证了一种使用质谱定量分析的方法，该方法在前人报道的方法基础上，通过样品的分级分离来定量葡萄酒中的单宁。使用内标是校正质谱信号变化的必要条件。在测试的不同化合物中，(-)-没食子儿茶素是唯一被证明是合适的内标物质，它可以实现主要橡木单宁的准确和个体定量。所开发的方法已用于检测和定量分析不同西班牙商业葡萄酒中的这些单宁，证明了其有用性。
