Alaeddini Mojgan, Fouladdel Shamileh, Etemad-Moghadam Shahroo, Azizi Ebrahim
Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran.
J Cancer Res Ther. 2011 Oct-Dec;7(4):427-32. doi: 10.4103/0973-1482.92009.
Fascin is an actin-bundling protein that has been shown to be overexpressed in various carcinomas, but the effect of doxorubicin on its protein and mRNA has not been investigated.
The aim of the present study is to evaluate any changes that may be induced by doxorubicin on the fascin molecule in a cancer cell line.
Experimental study.
The expression of fascin protein and mRNA in the human KB carcinoma cell line was evaluated by qRT-PCR, and immunocytochemistry following treatment with doxorubicin. Apoptosis was also analyzed using flow cytometry.
Group comparisons were performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test to determine differences between groups (P < 0.05).
Treatment with doxorubicin promoted a significant increase of fascin mRNA and decrease of fascin protein (P <0.05). Doxorubicin also induced apoptosis in the KB cell line.
Regarding the changes in the actin-bundling protein, fascin, which was found following treatment with doxorubicin, it can be hypothesized that this drug may possess antimigratory characteristics by influencing fascin. Further studies are suggested to clarify this theory.
Fascin是一种肌动蛋白成束蛋白，已证实在多种癌症中过度表达，但阿霉素对其蛋白质和mRNA的影响尚未得到研究。
本研究的目的是评估阿霉素对癌细胞系中Fascin分子可能诱导的任何变化。
实验研究。
用qRT-PCR以及阿霉素处理后的免疫细胞化学方法评估人KB癌细胞系中Fascin蛋白和mRNA的表达。还用流式细胞术分析了细胞凋亡情况。
采用单因素方差分析（ANOVA）进行组间比较，随后进行Tukey事后检验以确定组间差异（P<0.05）。
阿霉素处理促使Fascin mRNA显著增加，Fascin蛋白减少（P<0.05）。阿霉素还诱导KB细胞系发生凋亡。
鉴于阿霉素处理后发现肌动蛋白成束蛋白Fascin发生了变化，可以推测该药物可能通过影响Fascin而具有抗迁移特性。建议进一步研究以阐明这一理论。
