Department of Studies in Chemistry, University of Mysore, Mansagangotri, Mysore 570 006, India.
Environ Monit Assess. 2012 Oct;184(10):5983-8. doi: 10.1007/s10661-011-2395-x. Epub 2012 Jan 19.
A rapid, simple, accurate, and sensitive visible spectrophotometric method for the determination of trace amounts of hydrogen peroxide in acidic buffer medium is reported. The proposed method is based on the oxidative coupling of Ampyrone with dibenzazepin hydrochloride by hydrogen peroxide in the buffer medium of pH 4.0 which is catalyzed by ferrous iron. The blue-colored product formed with maximum absorption at 620 nm was found to be stable for 2 h. Beer's law is obeyed for hydrogen peroxide concentration in the range of 0.03-0.42 μg ml(-1). The optimum reaction conditions and other important optical parameters are reported. The molar absorptive and Sandell's sensitivity are found to be 5.89 × 10(4) mol(-1) cm(-1) and 0.57 g/cm(2), respectively. The interference due to diverse ions and complexing agents was studied. The method is successfully applied to the determination of hydrogen peroxide in green plants satisfactorily.
本文报道了一种在酸性缓冲介质中测定痕量过氧化氢的快速、简单、准确和灵敏的可见分光光度法。该方法基于在 pH 值为 4.0 的缓冲介质中，用过氧化氢将安吡酮与二苯并氮杂菲盐酸盐氧化偶联，该反应由二价铁离子催化。在 620nm 处具有最大吸收的蓝色产物在 2 小时内稳定。在 0.03-0.42μgml(-1)范围内，过氧化氢的浓度符合比尔定律。报道了最佳反应条件和其他重要的光学参数。摩尔吸光系数和桑德尔灵敏度分别为 5.89×10(4)mol(-1)cm(-1)和 0.57g/cm(2)。研究了多种离子和络合剂的干扰。该方法成功地应用于绿色植物中过氧化氢的测定。
