劳氏肉瘤病毒基因组感染性DNA拷贝的体外合成。

In vitro synthesis of infectious DNA copies of the Rous sarcoma virus genome.

作者信息

Mariage R, Hill M

出版信息

Intervirology. 1979;11(5):307-13. doi: 10.1159/000149050.

Abstract

Detergent-disrupted virions of Rous sarcoma virus can synthesize in vitro an infectious viral DNA. Infectivity of the DNA was determined in transfection assays at the endpoint dilution. It was found that the specific infectivity of the crude endogenous reaction product was 34 infectious units/microgram. The endogenous reaction yielded up to 8.5 x 10(-7) infectious units per biologically active input virion, i.e., nearly 0.1 -1 infectious molecules. The possible structure of these molecules is discussed.

摘要

经去污剂处理破坏的劳氏肉瘤病毒粒子能够在体外合成具有感染性的病毒DNA。通过终点稀释转染试验测定了DNA的感染性。结果发现，粗制内源性反应产物的比感染性为34个感染单位/微克。内源性反应每一个具有生物活性的输入病毒粒子可产生高达8.5×10⁻⁷个感染单位，即接近0.1 - 1个感染性分子。文中讨论了这些分子可能的结构。