Departament of Biochemistry, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
Inflammopharmacology. 2012 Dec;20(6):315-22. doi: 10.1007/s10787-011-0113-5. Epub 2012 Jan 20.
The present work reports the purification and partial characterization of an antibacterial lectin (EmaL) obtained from Eugenia malaccensis seeds as well as the evaluation of its effect in the daily topical treatment of repairing process of cutaneous wounds in mice.
The cutaneous wound was produced by the incision of the skin and use of lectin in the treatment of mice cutaneous wounds was evaluated. Surgical wounds were treated daily with a topical administration of EmaL and parameters such as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed.
A novel lectin, with a molecular mass of 14 kDa, was isolated from E. malaccensis using affinity chromatography. The lectin (EmaL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes; the lectin-induced rabbit erythrocyte agglutination was inhibited by glucose, casein, ovalbumin and fetuin. Also, Emal was very effective in the inhibition of bacterial growth, with the best inhibition results obtained for Staphylococcus aureus. Inflammatory signals such as edema and hyperemia were statistically less intense when EmaL was applied compared to the control. The histopathological analysis showed that the treated injured tissue presented reepithelialization (complete or partial) and areas of transition more evidenced than those of the control group, especially due to well organized pattern of collagen fibers presented in the granulation fibrous tissue.
Presented results are a preliminary indication of the pharmacological interest in using EmaL as antimicrobial agent and in the repairing process of cutaneous wounds.
本研究报告了从杨梅属种子中提取的一种具有抗菌活性的凝集素（EmaL）的纯化和部分特性，并评估了其在小鼠皮肤伤口日常局部治疗中的修复作用。
通过皮肤切口制备皮肤伤口，并评估凝集素在小鼠皮肤伤口治疗中的作用。每天对手术伤口进行局部 EmaL 处理，并分析水肿、充血、结痂、肉芽组织和疤痕组织以及伤口收缩等参数。
采用亲和层析法从杨梅属种子中分离出一种新型凝集素，分子量为 14 kDa。该凝集素（EmaL）可凝集戊二醛处理的兔和人红细胞；凝集素诱导的兔红细胞凝集可被葡萄糖、酪蛋白、卵清蛋白和胎球蛋白抑制。此外，EmaL 对细菌生长具有很强的抑制作用，对金黄色葡萄球菌的抑制效果最佳。与对照组相比，应用 EmaL 时，水肿和充血等炎症信号明显减轻。组织病理学分析表明，经处理的损伤组织具有再上皮化（完全或部分）和过渡区，比对照组更为明显，尤其是在肉芽纤维组织中呈现出胶原纤维排列有序。
这些结果初步表明，EmaL 作为一种抗菌剂和皮肤伤口修复过程具有药理学应用价值。
