Chen Ran, Choudhary Poonam, Schurr Ryan N, Bhattacharya Priyanka, Brown Jared M, Chun Ke Pu
Appl Phys Lett. 2012 Jan 2;100(1):13703-137034. doi: 10.1063/1.3672035. Epub 2012 Jan 5.
The physical interaction between a lipid vesicle and a silver nanoparticle (AgNP)-human serum albumin (HSA) protein "corona" has been examined. Specifically, the binding of AgNPs and HSA was analyzed by spectrophotometry, and the induced conformational changes of the HSA were inferred from circular dichroism spectroscopy. The fluidity of the vesicle, a model system for mimicking cell membrane, was found to increase with the increased exposure to AgNP-HSA corona, though less pronounced compared to that induced by AgNPs alone. This study offers additional information for understanding the role of physical forces in nanoparticle-cell interaction and has implications for nanomedicine and nanotoxicology.
已对脂质囊泡与银纳米颗粒（AgNP）-人血清白蛋白（HSA）蛋白质“冠层”之间的物理相互作用进行了研究。具体而言，通过分光光度法分析了AgNP与HSA的结合情况，并从圆二色光谱推断出HSA诱导的构象变化。发现作为模拟细胞膜的模型系统，囊泡的流动性随着与AgNP-HSA冠层接触的增加而增加，尽管与单独由AgNP诱导的情况相比不太明显。这项研究为理解物理力在纳米颗粒与细胞相互作用中的作用提供了更多信息，并对纳米医学和纳米毒理学具有启示意义。
