European Commission, Joint Research Centre, Institute for Health and Consumer Protection I-21027, Ispra (VA), Italy.
Nano Lett. 2011 Oct 12;11(10):4480-4. doi: 10.1021/nl202909s. Epub 2011 Sep 28.
We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD), we measure a decrease of 6 °C in the thermal unfolding of human serum albumin upon interaction with silver nanoparticles while this does not happen with gold. The use of SRCD allows measuring critical parameters on protein-nanoparticle interactions, and it will provide experimental data on the relative stability of key biological proteins for nanotoxicology.
我们测量了在与纳米粒子相互作用时，纳摩尔浓度下蛋白质的结构和稳定性变化。利用同步辐射圆二色性（SRCD），我们测量出人血清白蛋白与银纳米粒子相互作用时，热变性温度降低了 6°C，而与金纳米粒子相互作用时则没有这种情况。SRCD 的使用可以测量蛋白质-纳米粒子相互作用的关键参数，并为纳米毒理学中关键生物蛋白质的相对稳定性提供实验数据。
