Nomura K, Yoshida T
Shionogi Research Laboratories, Shionogi and Co. Ltd., Osaka, Japan.
FEMS Microbiol Lett. 1990 Aug;58(3):295-9. doi: 10.1111/j.1574-6968.1990.tb13992.x.
The Serratia marcescens SR50 chromosomal beta-lactamase gene (ampC) was cloned and sequenced. It contains 1128 nucleotides encoding a protein of 355 amino acids preceded by 21 amino acids which probably constitutes the signal peptide. The mature protein has a predicted molecular mass of 38,901 Da. About 40% of the amino acid sequence was identical among AmpC beta-lactamases resided in S. marcescens, Citrobacter freundii OS60, Escherichia coli K12 and Enterobacter cloacae P99. All of these enzymes are highly similar around the active site serine at the position 59 of the mature enzyme.
粘质沙雷氏菌SR50染色体β-内酰胺酶基因（ampC）被克隆并测序。它包含1128个核苷酸，编码一个由355个氨基酸组成的蛋白质，其前面还有21个氨基酸，这可能构成信号肽。成熟蛋白的预测分子量为38,901道尔顿。在粘质沙雷氏菌、弗氏柠檬酸杆菌OS60、大肠杆菌K12和阴沟肠杆菌P99中的AmpCβ-内酰胺酶之间，约40%的氨基酸序列相同。所有这些酶在成熟酶第59位的活性位点丝氨酸周围高度相似。
