Nakazawa T, Kimoto M, Abe M
Department of Microbiology, Yamaguchi University School of Medicine, Japan.
Gene. 1990 Sep 28;94(1):83-8. doi: 10.1016/0378-1119(90)90471-3.
A recombinant plasmid carrying the recA gene of Pseudomonas cepacia complements a recA mutation of Escherichia coli and restores UV and methylmethane sulfonate resistance, as well as recombinational proficiency. The predicted amino acid (aa) sequence of P. cepacia RecA (347 aa; Mr, 37256) is highly homologous to the RecA proteins from Thiobacillus ferrooxidans (74% aa homology), Pseudomonas aeruginosa (72%), E. coli (71%), Anabaena variabilis (61%), and Synechococcus sp. strains PCC7002 (59%). The transcription of the recA gene in P. cepacia and E. coli, which starts at almost the same site, was enhanced slightly by UV irradiation in the former and markedly in the latter bacteria. An SOS box characteristic to LexA-regulated promoters, along with the -10 and -35 consensus sequences, was found in the 5' upstream region of the P. cepacia recA gene.
携带洋葱伯克霍尔德菌recA基因的重组质粒可弥补大肠杆菌的recA突变，并恢复其对紫外线和甲磺酸甲酯的抗性以及重组能力。洋葱伯克霍尔德菌RecA的预测氨基酸（aa）序列（347个氨基酸；Mr，37256）与氧化亚铁硫杆菌的RecA蛋白（74%氨基酸同源性）、铜绿假单胞菌（72%）、大肠杆菌（71%）、多变鱼腥藻（61%）和聚球藻属菌株PCC7002（59%）高度同源。洋葱伯克霍尔德菌和大肠杆菌中recA基因的转录起始位点几乎相同，紫外线照射后，前者的转录略有增强，而后者则显著增强。在洋葱伯克霍尔德菌recA基因的5'上游区域发现了LexA调控启动子特有的SOS框以及-10和-35共有序列。
