斯氏假单胞菌recA基因表达的调控

Regulation of the expression of the Pseudomonas stutzeri recA gene.

作者信息

Vosman B, Rauch P J, Westerhoff H V, Hellingwerf K J

机构信息

Department of Microbiology & Biotechnology Centre, University of Amsterdam, The Netherlands.

出版信息

Antonie Van Leeuwenhoek. 1993 Jan;63(1):55-62. doi: 10.1007/BF00871732.

DOI:10.1007/BF00871732

PMID:8480993

Abstract

With the aid of recA-lacZ fusion strains, the in vivo regulation of the Pseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents, as well as with agents that interfere with DNA replication. For this induction, the presence of an active RecA protein is essential. Sequence analysis of the promoter region of the P. stutzeri recA gene showed that its open reading frame is preceded by an SOS-box, suggesting a regulation of its expression, similar to the regulation of recA expression in Escherichia coli.

摘要

借助recA-lacZ融合菌株，对斯氏假单胞菌recA基因的体内调控进行了研究。结果表明，该基因的表达可被多种DNA损伤剂以及干扰DNA复制的试剂诱导。对于这种诱导，活性RecA蛋白的存在至关重要。斯氏假单胞菌recA基因启动子区域的序列分析表明，其开放阅读框之前有一个SOS框，这表明其表达调控类似于大肠杆菌中recA表达的调控。