Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
ACS Appl Mater Interfaces. 2012 Feb;4(2):916-21. doi: 10.1021/am201576y. Epub 2012 Feb 9.
Molecularly imprinted polymers (MIPs) have frequently been employed as recognition elements in sensing applications, or for the controlled delivery of small molecule drugs. An equally important but less well studied application is the use of MIPs in the binding and immobilization of active enzymes. In this study, magnetic MIPs (MMIPs) recognizing the enzyme amylase were prepared using phase inversion of poly(ethylene-co-vinyl alcohol) (EVAL) solutions with 27-44 mol % ethylene in the presence of amylase. The size distribution, specific surface area, magnetization, and composition were characterized by dynamic light scattering (DLS), Brunauer-Emmett-Teller (BET) analysis, superconducting quantum interference devices (SQUID), and X-ray diffraction (XRD), respectively. The mean size of MMIPs was ~100 nm and the magnetization was 14.8 emu/g. The activities of both bound template and rebound enzyme was established by measuring glucose production via starch hydrolysis, at different temperatures, for MIPs with different compositions (wt % EVALs and mol % ethylene). The highest hydrolysis activity of MMIPs (obtained with 32 mol % ethylene) was found to be 1545.2 U/g. Finally, compared to the conventional catalysis process, MMIPs have the advantages of high surface area, suspension, easy removal from reaction, and rapid reload of enzyme. The good activity of amylase MMIPs persists after 50 cycles of starch hydrolysis.
分子印迹聚合物（MIPs）经常被用作传感应用中的识别元件，或用于控制小分子药物的递送。同样重要但研究较少的应用是将 MIPs 用于结合和固定活性酶。在这项研究中，在存在淀粉酶的情况下，使用具有 27-44mol%乙烯的聚（乙烯-co-醇）（EVAL）溶液的相反转制备了识别酶淀粉酶的磁性 MIPs（MMIPs）。通过动态光散射（DLS）、BET 分析、超导量子干涉器件（SQUID）和 X 射线衍射（XRD）分别对粒径分布、比表面积、磁化率和组成进行了表征。MMIPs 的平均粒径约为 100nm，磁化率为 14.8emu/g。通过测量淀粉水解过程中不同温度下的葡萄糖产量，确定了不同组成（wt%EVAL 和 mol%乙烯）的 MIPs 中结合模板和回弹酶的活性。发现具有 32mol%乙烯的 MMIPs 的水解活性最高，为 1545.2U/g。最后，与传统的催化过程相比，MMIPs 具有高表面积、悬浮、易于从反应中去除以及快速重新加载酶的优点。在 50 次淀粉水解循环后，淀粉酶 MMIPs 仍保持良好的活性。
