Seversike Thomas M, Ray Jeffery D, Shultz Jeffry L, Purcell Larry C
Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA.
Theor Appl Genet. 2008 Jul;117(2):143-7. doi: 10.1007/s00122-008-0759-6. Epub 2008 Apr 8.
The seven-leaflet character of soybean [Glycine max L. (Merr.)] is a single recessive trait conditioned by the lf ( 2 ) gene. The lf ( 2 ) gene is located on linkage group (LG) 16 of the classical soybean genetic map, but it has not been placed on the molecular map. The objective of this research was to identify the location of the lf ( 2 ) gene on the soybean molecular map using simple sequence repeat (SSR) markers. A backcross breeding method was used to create three- and seven-leaflet near-isogenic lines in genetic backgrounds of 'Traill', 'MN1401', and 'MN1801'. Eight mapping populations were derived from eight single heterozygous Lf ( 2 ) lf ( 2 ) plants. A total of 482 SSR markers that covered approximately every 10-20 cM of all soybean molecular LG were used to screen the mapping populations for polymorphisms. For the 115 SSRs that were identified as polymorphic, possible linkage between the lf ( 2 ) gene and the polymorphic SSR markers was determined. One SSR marker from the LG B1, Sat_272, was linked (LOD > 4.0) to the lf ( 2 ) gene in the Traill and MN1401 derived populations, with map distances ranging from 2.8 to 11.2 cM. Two additional markers (a SSR, Sat_270 and a SNP, A588c) located on LG B1 were also polymorphic and were identified as linked to the lf ( 2 ) gene in one of the populations. This research was successful in mapping the lf ( 2 ) gene to LG B1 of the soybean molecular map and therefore, provides evidence that molecular LG B1 corresponds to classical LG 16.
大豆[Glycine max L. (Merr.)]的七叶特性是由lf(2)基因控制的单隐性性状。lf(2)基因位于经典大豆遗传图谱的连锁群(LG) 16上，但尚未定位到分子图谱上。本研究的目的是利用简单序列重复(SSR)标记确定lf(2)基因在大豆分子图谱上的位置。采用回交育种方法，在‘Traill’、‘MN1401’和‘MN1801’的遗传背景下创建三叶和七叶近等基因系。8个作图群体来自8株单杂合Lf(2)lf(2)植株。总共482个SSR标记覆盖了大豆所有分子连锁群大约每10 - 20 cM的区域，用于筛选作图群体的多态性。对于鉴定为多态性的115个SSR，确定了lf(2)基因与多态性SSR标记之间可能的连锁关系。来自连锁群B1的一个SSR标记Sat_272在Traill和MN1401衍生群体中与lf(2)基因连锁(LOD > 4.0)，图距范围为2.8至11.2 cM。位于连锁群B1上的另外两个标记(一个SSR，Sat_270和一个SNP，A588c)也具有多态性，并在其中一个群体中被鉴定为与lf(2)基因连锁。本研究成功地将lf(2)基因定位到大豆分子图谱的连锁群B1上，因此，提供了分子连锁群B1对应于经典连锁群16的证据。
