Department of Periodontology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
J Clin Periodontol. 2012 Apr;39(4):342-9. doi: 10.1111/j.1600-051X.2011.01848.x. Epub 2012 Jan 26.
Periodontal disease is highly prevalent and severe in diabetic patients, and is considered one of the diabetic complications. To elucidate how periodontitis progresses in diabetes, we examined an animal model of periodontitis in diabetic rats.
Two weeks after the induction of diabetes by streptozotocin, surgical nylon thread was ligated around the cervical portion of the unilateral maxillary second molar to induce periodontitis. Periodontitis was evaluated 2 weeks after the ligation by gingival blood flow, mRNA expressions, Western blot analysis, histological examination and micro CT.
Ligation-induced severe periodontitis in the diabetic rats, which was apparently shown by the increase of TNF-α and iNOS mRNA expressions and inflammatory cell infiltration in the gingiva and alveolar bone loss. The number of nitrotyrosine, a footprint of nitrosative stress, -positive cells was significantly higher in the periodontitis of the diabetic rats compared with that in the normal rats. Western blot analysis confirmed that the nitrotyrosine was increased in the periodontitis of the diabetic rats.
This is the first study to confirm increased nitrosative stress due to periodontitis in diabetic rats. Nitrosative stress may play a crucial role in the exacerbation of periodontitis in diabetic patients.
牙周病在糖尿病患者中普遍且严重，被认为是糖尿病的并发症之一。为了阐明牙周炎在糖尿病中的进展情况，我们检查了糖尿病大鼠牙周炎的动物模型。
用链脲佐菌素诱导糖尿病 2 周后，用手术尼龙线将单侧上颌第二磨牙颈部结扎，诱发牙周炎。结扎后 2 周，通过牙龈血流、mRNA 表达、Western blot 分析、组织学检查和 micro CT 评估牙周炎。
糖尿病大鼠的结扎诱导了严重的牙周炎，这明显表现为 TNF-α 和 iNOS mRNA 表达的增加以及牙龈和牙槽骨丧失处的炎症细胞浸润。与正常大鼠相比，糖尿病大鼠牙周炎中硝基酪氨酸（一种硝化应激的标志物）阳性细胞的数量明显增加。Western blot 分析证实糖尿病大鼠牙周炎中硝基酪氨酸增加。
这是第一项证实糖尿病大鼠牙周炎中硝化应激增加的研究。硝化应激可能在糖尿病患者牙周炎的恶化中起关键作用。
