School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, People's Republic of China.
Department of Gastroenterology, Second Clinical Hospital of North Sichuan Medical College, Nanchong Central Hospital, Nanchong, People's Republic of China.
Redox Rep. 2021 Dec;26(1):197-202. doi: 10.1080/13510002.2021.2004047.
As a chronic autoimmune disease, rheumatoid arthritis (RA) is related to oxidative stress, which may lead to the occurrence and persistence of inflammation in RA. The purpose of this study is to evaluate the potential antioxidant effect of triptolide in collagen-induced arthritis (CIA) rat model.
We examined the severity of arthritis, levels of local and systemic oxidative stress, periarticular bone erosion and weight of organs in CIA rats treated with triptolide.
We found that triptolide decreased the paw thickness and clinical arthritis score, significantly. The mRNA expression and activity of myeloperoxidase and inducible nitric oxide synthase were remarkably decreased in the paws of the CIA rats after triptolide treatment. Triptolide significantly inhibited the levels of nitrite and nitrate in serum, as well as the urinary level of dityrosine. Triptolide treatment also markedly increased bone volume of tibia, but suppressed epiphyseal plate thickness of both femur and tibia. In addition, there was no significant difference in the weight of organs after the therapy, except decreased spleen weight.
These results suggested that the local and systemic oxidative stress was enhanced in the CIA rats and the therapeutic dose of triptolide had a definite antioxidant effect.
类风湿关节炎（RA）是一种慢性自身免疫性疾病，与氧化应激有关，可能导致 RA 炎症的发生和持续。本研究旨在评估雷公藤内酯醇在胶原诱导性关节炎（CIA）大鼠模型中的潜在抗氧化作用。
我们检查了雷公藤内酯醇治疗 CIA 大鼠后关节炎的严重程度、局部和全身氧化应激水平、关节周围骨侵蚀和器官重量。
我们发现雷公藤内酯醇可显著降低爪厚度和临床关节炎评分。雷公藤内酯醇治疗后，CIA 大鼠爪中髓过氧化物酶和诱导型一氧化氮合酶的 mRNA 表达和活性明显降低。雷公藤内酯醇显著抑制血清中亚硝酸盐和硝酸盐水平以及尿中二酪氨酸水平。雷公藤内酯醇治疗还显著增加了胫骨的骨量，但抑制了股骨和胫骨的骺板厚度。此外，治疗后除脾脏重量减轻外，各器官重量无明显差异。
这些结果表明 CIA 大鼠的局部和全身氧化应激增强，雷公藤内酯醇的治疗剂量具有明确的抗氧化作用。
