Vítovec J, Koudela B
Institute of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Folia Parasitol (Praha). 1990;37(3):193-9.
It was confirmed, after experimental infection of 24 weaned pigs with different doses (200,000 and 4 mil.) of Eimeria debliecki oocysts that the developmental cycle of E. debliecki occurs in the anterior jejunum and after a high dose of oocysts also in the duodenum and anterior parts of the middle jejunum. Pathological changes characterized by a light atrophy of the villi, scarcely dispersed minute erosions of the epithelium in upper parts of the mucosa and an inflammatory response in the propria of the anterior jejunum were found in the area of the largest occurrence of developmental stages of E. debliecki (from 50 cm to 100 cm from the pylorus). An inflammatory infiltrate in the propria of the anterior jejunum from 3 DPI to 5 DPI contained a conspicuously large number of plasma cells with Russell bodies. Cellular changes were detected only in enterocytes with developmental stages of E. debliecki. Changes of the microvillous zone were observed in infected enterocytes; dilated mitochondria, free ribosomes and an increased number of residual bodies were found in the cytoplasm of enterocytes. The degree of the cellular changes of enterocytes was dependent on the maturity of the developmental stage of E. debliecki. Based on pathological changes observed by the use of light and electron microscopy, the coccidium E. debliecki is considered to be pathogenic for weaned pigs in spite it does not provoke a clinical infection.
在用不同剂量（200,000个和400万个）的德氏艾美耳球虫卵囊对24头断奶仔猪进行实验性感染后，证实德氏艾美耳球虫的发育周期发生在前段空肠，在高剂量卵囊感染后，十二指肠和空肠中段前部也会出现。在德氏艾美耳球虫发育阶段出现最多的区域（距幽门50厘米至100厘米处），发现了以绒毛轻度萎缩、黏膜上部上皮有少量散在的微小糜烂以及前段空肠固有层有炎症反应为特征的病理变化。从感染后第3天到第5天，前段空肠固有层的炎性浸润中含有大量带有拉塞尔小体的浆细胞。仅在有德氏艾美耳球虫发育阶段的肠上皮细胞中检测到细胞变化。在受感染的肠上皮细胞中观察到微绒毛区的变化；在肠上皮细胞的细胞质中发现线粒体扩张、游离核糖体和残余小体数量增加。肠上皮细胞的细胞变化程度取决于德氏艾美耳球虫发育阶段的成熟度。基于光学显微镜和电子显微镜观察到的病理变化，尽管德氏艾美耳球虫不会引发临床感染，但仍被认为对断奶仔猪具有致病性。
