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优化光照强度和硝酸盐供应协同增强节旋藻中糖原的生成。

Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply.

机构信息

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan.

出版信息

Bioresour Technol. 2012 Mar;108:211-5. doi: 10.1016/j.biortech.2012.01.004. Epub 2012 Jan 9.

DOI:10.1016/j.biortech.2012.01.004
PMID:22277210
Abstract

Arthrospira (Spirulina) platensis, a fast-growing halophilic cyanobacterium able to accumulate glycogen, was investigated for its feasibility to serve as feedstock for fermentative production of biofuels and chemicals. The culture conditions most appropriate for glycogen production were identified. Glycogen production was maximized by the depleting nitrate source under a high light intensity of 700 μmol photons m(-2) s(-1). With optimal control of both light intensity and nitrate supply, glycogen production of A. platensis reached nearly 1.03 g L(-1) (a glycogen productivity of 0.29 g L(-1) d(-1)), which is, to the best of our knowledge, the highest α-polyglucan (glycogen or starch) production performance ever reported in microalgae. The outcome of this work supports A. platensis as a promising carbohydrate source for biorefinery.

摘要

节旋藻(螺旋藻)是一种快速生长的嗜盐蓝藻,能够积累糖原,本研究探讨了其作为发酵生产生物燃料和化学品原料的可行性。确定了最适合生产糖原的培养条件。在 700 μmol 光子 m(-2) s(-1) 的高光强下耗尽硝酸盐源可实现最大糖原生产。通过对光强度和硝酸盐供应的最佳控制,节旋藻的糖原生产达到近 1.03 g L(-1)(糖原生产率为 0.29 g L(-1) d(-1)),据我们所知,这是微藻中报道的最高α-多糖(糖原或淀粉)生产性能。这项工作的结果支持节旋藻作为生物炼制中有前途的碳水化合物来源。

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