Department of Biology, Eskişehir Osmangazi University, Eskişehir, Turkey.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(12):1938-47. doi: 10.1080/03601234.2012.676517.
Chlorinated compounds constitute an important class of xenobiotics. Crude laccase was produced using Trametes versicolor ATCC (200801) in potato dextrose broth, with wheat bran as an inducing medium, and its ability to dechlorinate eight compounds was determined. The compounds were 2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,6-dichlorophenol, 2,4,5-trichlorophenol, 2,4,6-trichlorophenol, heptachlor and pentachlorophenol. A range of parameters for the dechlorination of some compounds was tested, including incubation period, pH, initial substrate concentration, temperature, and enzyme quantity. The oxygen consumption was determined during each dechlorination process, under pre-determined optimum conditions. The changes in chemical structure of the compounds were also determined, by using FTIR analysis, following dechlorination of test chlorophenolics. Strong interactions were found to lead to the reactivity of hydroxyl groups in some cases and chlorine atoms were released from the benzene ring. The changes in compound toxicity were monitored before and after enzymatic treatment, using Microtox. Quantitative structure-activity relationships for the toxicity of the chlorinated compounds were developed. Consequently, the toxic activity of the test compounds was controlled by electrophilic index and electronic properties.
氯代化合物构成了一类重要的外源性化合物。利用糙皮侧耳（Trametes versicolor ATCC 200801）在土豆葡萄糖肉汤中生产粗漆酶，并以麦麸作为诱导培养基，测定其脱氯八种化合物的能力。这些化合物分别为 2-氯苯酚、4-氯苯酚、2,4-二氯苯酚、2,6-二氯苯酚、2,4,5-三氯苯酚、2,4,6-三氯苯酚、七氯和五氯苯酚。对一些化合物的脱氯进行了一系列参数的测试，包括孵育时间、pH 值、初始底物浓度、温度和酶量。在每个脱氯过程中，在预先确定的最佳条件下，测定氧消耗。通过 FTIR 分析，在脱氯测试氯酚类物质后，确定了化合物化学结构的变化。发现强相互作用导致某些情况下羟基的反应性增强，并且氯原子从苯环上释放出来。在酶处理前后，使用 Microtox 监测化合物毒性的变化。建立了氯化化合物毒性的定量构效关系。因此，测试化合物的毒性活性受亲电指数和电子性质控制。
