Chang Seok-Woo, Lee So-Youn, Kum Kee-Yeon, Kim Eun-Cheol
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Department of Maxillofacial Tissue Regeneration, Research Center for Tooth and Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
J Endod. 2014 Jan;40(1):113-8. doi: 10.1016/j.joen.2013.09.036. Epub 2013 Nov 1.
The aim of this study was to compare the biocompatibility and odontogenic potential of newly developed Bioaggregate (BA) and Micromega MTA (MMTA) with ProRoot MTA (PMTA) and intermediate restorative material (IRM) by using human dental pulp cells.
Biocompatibility was assessed by an 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide assay and scanning electron microscopy. Differentiation was evaluated by alkaline phosphatase (ALP) activity, alizarin red staining, and reverse transcriptase-polymerase chain reaction for the maker genes. The levels of inflammatory mediators and cytokines were measured by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay.
PMTA, BA, and MMTA exhibited equally good biocompatibility, whereas IRM showed cytotoxicity compared with these materials. PMTA, BA, and MMTA increased the ALP activity, promoted mineralization nodule formation, and enhanced the mRNA expression level of the osteogenic/odontogenic markers (ALP, osteopontin, osteocalcin, dentin sialophosphoprotein, and dentin matrix protein-1) compared with IRM. The levels of proinflammatory mediators and proinflammatory cytokines were lower in PMTA, BA, and MMTA compared with the IRM group.
Collectively, the biocompatibility, odontogenic potentials, and inflammatory response of BA and MMTA are equal to those of PMTA and superior to those of IRM.
本研究的目的是通过使用人牙髓细胞，比较新开发的生物聚集体（BA）和微米加MTA（MMTA）与ProRoot MTA（PMTA）和中间修复材料（IRM）的生物相容性和牙源性潜力。
通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐试验和扫描电子显微镜评估生物相容性。通过碱性磷酸酶（ALP）活性、茜素红染色以及对标记基因进行逆转录聚合酶链反应来评估分化情况。通过逆转录聚合酶链反应和酶联免疫吸附测定法测量炎症介质和细胞因子的水平。
PMTA、BA和MMTA表现出同样良好的生物相容性，而与这些材料相比，IRM显示出细胞毒性。与IRM相比，PMTA、BA和MMTA提高了ALP活性，促进了矿化结节的形成，并增强了成骨/牙源性标记物（ALP、骨桥蛋白、骨钙素、牙本质涎磷蛋白和牙本质基质蛋白-1）的mRNA表达水平。与IRM组相比，PMTA、BA和MMTA中促炎介质和促炎细胞因子的水平较低。
总体而言，BA和MMTA的生物相容性、牙源性潜力和炎症反应与PMTA相当，且优于IRM。
