UNESP - São Paulo State University, Araraquara School of Dentistry, Department of Restorative Dentistry, Araraquara, São Paulo, Brazil.
University of Malta, Faculty of Dental Surgery, Department of Restorative Dentistry, Msida, Malta.
Int Endod J. 2017 Dec;50 Suppl 2:e31-e39. doi: 10.1111/iej.12780. Epub 2017 May 2.
To evaluate the biocompatibility and mineralized nodule formation of an experimental tricalcium silicate cement with tantalum oxide (TSC/Ta O ) as radiopacifier, Neo MTA Plus (Avalon Biomed Inc., Bradenton, FL, USA) and MTA (Angelus, Londrina, PR, Brazil) on human osteoblast-like cells (Saos-2).
Biocompatibility was evaluated by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and neutral red (NR) assays, after exposure of Saos-2 to cement extracts at 1 : 1, 1 : 2, 1 : 4 and 1 : 8 dilutions for 24 h. Bioactivity was evaluated by alkaline phosphatase (ALP) activity, and calcium deposits were detected with alizarin red staining (ARS). Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post-test (α = 0.05).
The MTT assay revealed lower cytotoxicity for NEO and MTA (P < 0.05), and higher for TSC/Ta O at 1 : 1 and 1 : 2 dilutions when compared to serum-free medium - control (P > 0.05). At 1 : 4 dilution, the TSC/Ta O cytotoxicity was similar to the control (P > 0.05). At 1 : 8 dilution, cell viability was significantly greater than the control (P < 0.05). Saos-2 cell viability performed using the NR assay at all dilutions revealed no cytotoxic effect of MTA, NEO and TSC/Ta O . ALP activity at 1 and 3 days was similar to the control (P > 0.05). TSC/Ta O had significantly greater ALP activity at 7 days when compared with the control (P < 0.05). All materials induced the production of mineralized nodules, and NEO produced significantly more mineralized nodules than MTA and TSC/Ta O (P < 0.05).
Neo MTA Plus and TSC/Ta O were biocompatible and induced ALP activity in Saos-2 cells. Both materials induced mineralized nodule formation by Saos-2 with Neo MTA Plus producing significantly more.
评估含氧化钽的硅酸三钙水泥（TSC/TaO）作为放射显影剂的生物相容性和矿化结节形成能力，与 Neo MTA Plus（Avalon Biomed Inc.，美国布拉登顿）和 MTA（Angelus，巴西隆德里纳）相比，对人成骨样细胞（Saos-2）的影响。
通过 3-（4,5-二甲基噻唑-2,5-二苯基）-2,5-二苯基四氮唑溴盐（MTT）和中性红（NR）测定法，在 1:1、1:2、1:4 和 1:8 稀释度下，将 Saos-2 暴露于水泥提取物中 24 小时，评估生物相容性。通过碱性磷酸酶（ALP）活性评估生物活性，并通过茜素红染色（ARS）检测钙沉积物。采用方差分析和 Bonferroni 或 Tukey 后检验（α=0.05）进行统计分析。
MTT 测定法显示，与无血清培养基对照（P>0.05）相比，NEO 和 MTA 的细胞毒性较低（P<0.05），而 TSC/TaO 在 1:1 和 1:2 稀释度时的细胞毒性较高（P>0.05）。在 1:4 稀释度时，TSC/TaO 的细胞毒性与对照相似（P>0.05）。在 1:8 稀释度时，细胞活力明显大于对照（P<0.05）。使用 NR 测定法在所有稀释度下测定 Saos-2 细胞活力，结果显示 MTA、NEO 和 TSC/TaO 均无细胞毒性作用。在第 1 和 3 天的 ALP 活性与对照相似（P>0.05）。与对照相比，TSC/TaO 在第 7 天的 ALP 活性显著更高（P<0.05）。所有材料均诱导矿化结节的产生，且 NEO 产生的矿化结节明显多于 MTA 和 TSC/TaO（P<0.05）。
Neo MTA Plus 和 TSC/TaO 具有生物相容性，并诱导 Saos-2 细胞中的 ALP 活性。两种材料均通过 Saos-2 诱导矿化结节形成，其中 Neo MTA Plus 产生的矿化结节明显更多。
