Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi’an, 710032, PR China.
Dent Mater J. 2012 Feb 3;31(1):150-6. doi: 10.4012/dmj.2011-180. Epub 2012 Jan 21.
Pretreatment of dentin using colloidal platinum nanoparticles (CPtN) can enhance the bond strength of dentin adhesives. However, the combination of CPtN, which is negatively charged, with cationic monomer-containing adhesive may reduce the antibacterial activity of the original material. Thus, the purpose of this study was to assess the effect of CPtN on the bactericidal activity of two cationic antibacterial monomers, 12-methacryloyloxydodecylpyridinium bromide (MDPB) and methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB). The rapid killing effects of the two monomers against planktonic or attached Streptococcus mutans in the presence or absence of CPtN were examined by viable cell counts. The measurement of minimum inhibitory and bactericidal concentrations demonstrated that CPtN up to 2.5 mM has no antibacterial activity. In the absence of CPtN, rapid killing of both planktonic and attached Streptococcus mutans were achieved by the two cationic monomers. Combination with 0.1 mM CPtN did not reduce the bactericidal effects of the two monomers, indicating that CPtN may be used as a pretreatment with antibacterial adhesives.
使用胶体铂纳米粒子（CPtN）预处理牙本质可以提高牙本质黏结剂的粘结强度。然而，带负电荷的 CPtN 与含阳离子单体的黏合剂结合可能会降低原始材料的抗菌活性。因此，本研究的目的是评估 CPtN 对两种阳离子抗菌单体 12-甲氧基十二烷基溴化吡啶（MDPB）和甲基丙烯酰氧乙基十六烷基二甲基氯化铵（DMAE-CB）杀菌活性的影响。通过活菌计数法检测了两种单体在存在或不存在 CPtN 时对浮游或附着变形链球菌的快速杀菌作用。最低抑菌浓度和杀菌浓度的测定表明，高达 2.5 mM 的 CPtN 没有抗菌活性。在不存在 CPtN 的情况下，两种阳离子单体均可迅速杀死浮游和附着的变形链球菌。与 0.1 mM CPtN 联合使用不会降低两种单体的杀菌作用，这表明 CPtN 可作为具有抗菌作用的黏合剂的预处理剂。
