Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
J Dent. 2013 Jun;41(6):504-13. doi: 10.1016/j.jdent.2013.03.011. Epub 2013 Apr 10.
The objectives of this study were to develop bonding agent containing a new antibacterial monomer dimethylaminododecyl methacrylate (DMADDM) as well as nanoparticles of silver (NAg) and nanoparticles of amorphous calcium phosphate (NACP), and to investigate the effects of water-ageing for 6 months on dentine bond strength and anti-biofilm properties for the first time.
Four bonding agents were tested: Scotchbond Multi-Purpose (SBMP) Primer and Adhesive control; SBMP+5% DMADDM; SBMP+5% DMADDM+0.1% NAg; and SBMP+5% DMADDM+0.1% NAg with 20% NACP in adhesive. Specimens were water-aged for 1d and 6 months at 37°C. Then the dentine shear bond strengths were measured. A dental plaque microcosm biofilm model was used to inoculate bacteria on water-aged specimens and to measure metabolic activity, colony-forming units (CFUs), and lactic acid production.
Dentine bond strength showed a 35% loss in 6 months of water-ageing for SBMP control (mean±sd; n=10); in contrast, the new antibacterial bonding agents showed no strength loss. The DMADDM-NAg-NACP containing bonding agent imparted a strong antibacterial effect by greatly reducing biofilm viability, metabolic activity and acid production. The biofilm CFU was reduced by more than two orders of magnitude, compared to SBMP control. Furthermore, the DMADDM-NAg-NACP bonding agent exhibited a long-term antibacterial performance, with no significant difference between 1d and 6 months (p>0.1).
Incorporating DMADDM-NAg-NACP in bonding agent yielded potent and long-lasting antibacterial properties, and much stronger bond strength after 6 months of water-ageing than a commercial control. The new antibacterial bonding agent is promising to inhibit biofilms and caries at the margins. The method of DMADDM-NAg-NACP incorporation may have a wide applicability to other adhesives, cements and composites.
本研究旨在开发一种新型抗菌单体二甲基氨基十二烷基甲基丙烯酸酯（DMADDM）和纳米银（NAg）以及纳米无定形磷酸钙（NACP）的粘结剂，并首次研究水老化 6 个月对牙本质粘结强度和抗生物膜性能的影响。
共测试了 4 种粘结剂：Scotchbond Multi-Purpose (SBMP) Primer 和 Adhesive 对照组；SBMP+5% DMADDM；SBMP+5% DMADDM+0.1% NAg；以及 SBMP+5% DMADDM+0.1% NAg+20% NACP 在粘结剂中。将样本在 37°C 下分别水老化 1d 和 6 个月。然后测量牙本质剪切粘结强度。使用牙菌斑微宇宙生物膜模型在水老化样本上接种细菌，并测量代谢活性、菌落形成单位（CFUs）和乳酸产生。
SBMP 对照组在 6 个月的水老化中，牙本质粘结强度损失了 35%（平均值±标准差；n=10）；相比之下，新型抗菌粘结剂没有出现强度损失。含 DMADDM-NAg-NACP 的粘结剂通过极大地降低生物膜活力、代谢活性和酸产生，赋予了强大的抗菌效果。与 SBMP 对照组相比，生物膜 CFU 减少了两个数量级以上。此外，DMADDM-NAg-NACP 粘结剂表现出长期的抗菌性能，1d 和 6 个月之间没有显著差异（p＞0.1）。
将 DMADDM-NAg-NACP 掺入粘结剂中，可获得强大且持久的抗菌性能，并在水老化 6 个月后获得比商业对照更强的粘结强度。新型抗菌粘结剂有望抑制边缘的生物膜和龋齿。DMADDM-NAg-NACP 掺入方法可能具有广泛的适用性，可以应用于其他粘结剂、水泥和复合材料。
