Eiampongpaiboon Trinuch, Chung Whasun O, Bryers James D, Chung Kwok-Hung, Chan Daniel C N
Department of Prosthodontics, Mahidol UniversityBangkokThailand.
Department of Oral Health Sciences, University of WashingtonSeattleWAUSA.
Acta Biomater Odontol Scand. 2015 Sep 18;1(2-4):51-58. doi: 10.3109/23337931.2015.1084883. eCollection 2015 Dec.
: Gram-positive cariogenic bacteria are etiological agents in dental caries; therefore, strategies to inhibit these bacteria to reduce the incident of this disease have intensified. In this study, we investigated antibacterial activities of titanates and gold-titanates against (Lc) and (Sm). : Monosodium titanate (MST), nanomonosodium titanate (nMST) and amorphous peroxo-titanate (APT), which are inorganic compounds with high-binding affinity for specific metal ions, were used. Total bacterial proteins were measured to represent bacterial cell mass after 24 h incubation with gold-titanates. We further examined the effect of nMST-Au(III) concentrations (10,200,400 mg/L) on Lc and Sm cell viability over time via Live/Dead fluorescent staining and colony forming units (CFUs). Transmission electron microscopy (TEM) was used to determine specific locations on the bacterial cells affected by the nMST-Au(III). : We found all gold-titanates and APT alone reduced bacterial protein for Lc ( value <0.001) while only MST-Au(III) and nMST-Au(III) affected Sm growth ( value <0.001). Overall, nMST-Au(III) showed the most effectiveness against both Lc and Sm at 400 mg/L. The Live/Dead staining showed all concentrations of nMST-Au(III) affected Lc growth but only 200 and 400 mg/L nMST-Au(III) interrupted Sm growth. The growth curves based on CFUs/mL showed all nMST-Au(III) concentrations affected growth of both Lc and Sm TEM images showed nMST-Au(III) attached to Lc and Sm cell wall and were internalized into both cells: nMST-Au(III) demonstrated potential antimicrobial activity against Gram-positive cariogenic bacteria. These results support further development of nMST-Au(III) as a potential novel material to prevent dental caries.
革兰氏阳性致龋菌是龋齿的病原体；因此，抑制这些细菌以减少该疾病发病率的策略得到了加强。在本研究中，我们研究了钛酸盐和金钛酸盐对变形链球菌（Lc）和远缘链球菌（Sm）的抗菌活性。使用了钛酸钠（MST）、纳米钛酸钠（nMST）和无定形过氧钛酸盐（APT），它们是对特定金属离子具有高结合亲和力的无机化合物。在与金钛酸盐孵育24小时后，测量总细菌蛋白以代表细菌细胞质量。我们通过活/死荧光染色和菌落形成单位（CFU）进一步研究了nMST-Au（III）浓度（10、200、400mg/L）随时间对Lc和Sm细胞活力的影响。透射电子显微镜（TEM）用于确定受nMST-Au（III）影响的细菌细胞上的特定位置。我们发现所有金钛酸盐和单独的APT都降低了Lc的细菌蛋白（P值<0.001），而只有MST-Au（III）和nMST-Au（III）影响Sm的生长（P值<0.001）。总体而言，nMST-Au（III）在400mg/L时对Lc和Sm均显示出最有效的抗菌活性。活/死染色显示所有浓度的nMST-Au（III）均影响Lc的生长，但只有200和400mg/L的nMST-Au（III）会干扰Sm的生长。基于CFU/mL的生长曲线显示，所有nMST-Au（III）浓度均影响Lc和Sm的生长。TEM图像显示nMST-Au（III）附着在Lc和Sm细胞壁上并被内化到两个细胞中：nMST-Au（III）对革兰氏阳性致龋菌具有潜在的抗菌活性。这些结果支持进一步开发nMST-Au（III）作为预防龋齿的潜在新型材料。
