Institute of Health & Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, Queensland 4059, Australia.
Cell Tissue Res. 2012 Mar;347(3):603-12. doi: 10.1007/s00441-011-1298-z. Epub 2012 Jan 26.
There is a growing need for successful bone tissue engineering strategies and advanced biomaterials that mimic the structure and function of native tissues carry great promise. Successful bone repair approaches may include an osteoconductive scaffold, osteoinductive growth factors, cells with an osteogenic potential and capacity for graft vascularisation. To increase osteoinductivity of biomaterials, the local combination and delivery of growth factors has been developed. In the present study we investigated the osteogenic effects of calcium phosphate (CaP)-coated nanofiber mesh tube-mediated delivery of BMP-7 from a PRP matrix for the regeneration of critical sized segmental bone defects in a small animal model. Bilateral full-thickness diaphyseal segmental defects were created in twelve male Lewis rats and nanofiber mesh tubes were placed around the defect. Defects received either treatment with a CaP-coated nanofiber mesh tube (n = 6), an un-coated nanofiber mesh tube (n=6) a CaP-coated nanofiber mesh tube with PRP (n=6) or a CaP-coated nanofiber mesh tube in combination with 5 μg BMP-7 and PRP (n = 6). After 12 weeks, bone volume and biomechanical properties were evaluated using radiography, microCT, biomechanical testing and histology. The results demonstrated significantly higher biomechanical properties and bone volume for the BMP group compared to the control groups. These results were supported by the histological evaluations, where BMP group showed the highest rate of bone regeneration within the defect. In conclusion, BMP-7 delivery via PRP enhanced functional bone defect regeneration, and together these data support the use of BMP-7 in the treatment of critical sized defects.
对于成功的骨组织工程策略和仿生天然组织结构和功能的先进生物材料的需求日益增长，这些材料具有很大的应用前景。成功的骨修复方法可能包括具有骨诱导性的支架、骨诱导生长因子、具有成骨潜能和移植物血管化能力的细胞。为了提高生物材料的骨诱导能力，已经开发了局部组合和递送生长因子的方法。在本研究中，我们研究了在小动物模型中，通过 PRP 基质介导 BMP-7 的钙磷（CaP）涂层纳米纤维网管传递，对临界尺寸节段性骨缺损的成骨作用。在 12 只雄性 Lewis 大鼠双侧股骨干全层节段性缺损，并在缺损周围放置纳米纤维网管。缺陷分别接受以下处理：CaP 涂层纳米纤维网管（n=6）、未涂层纳米纤维网管（n=6）、CaP 涂层纳米纤维网管+PRP（n=6）或 CaP 涂层纳米纤维网管结合 5μg BMP-7 和 PRP（n=6）。12 周后，通过 X 射线、microCT、生物力学测试和组织学评估评估骨体积和生物力学性能。结果表明，BMP 组的生物力学性能和骨体积明显高于对照组。组织学评估结果支持了这一结果，BMP 组在缺陷内显示出最高的骨再生率。总之，通过 PRP 递送 BMP-7 增强了功能性骨缺损的再生，这些数据支持将 BMP-7 用于治疗临界尺寸的缺陷。
