Liu Liyan, Sun Changhao, Wang Cheng, Li Ying
School of Public Health, Harbin Medical University, Harbin 150081, China.
Wei Sheng Yan Jiu. 2011 Nov;40(6):756-8.
To develop a HPLC method for the determination of serum vitamin B1 (VB1) and vitamin B2 (VB2) simultaneously.
Serum samples were pretreated at ambient temperature on a C18 reversed phase chromatographic column, methanol plus phosphate buffer was used for mobile phase, vitamin B1 and B2 contents of serum samples eluted from a Sep-park C18 column were detected with a photodiode array detector. The influences of retention time, the volume and the proportion of eluent on the recovery of samples were investigated.
Recoveries were ranged from 82.67 to 96.89%. The coefficient of variation was < 10%. The linearity and correlation coefficient was 0.9985 and 0.9995, respectively. The contents of serum VB1 and VB2 in obese people were lower than those in healthy controls.
A HPLC method of determining serum VB1 and VB2 was established. Serum VB1 and VB2 level in obese people was low.
建立同时测定血清维生素B1（VB1）和维生素B2（VB2）的高效液相色谱法。
血清样品在室温下于C18反相色谱柱上进行预处理，以甲醇加磷酸盐缓冲液为流动相，用光电二极管阵列检测器检测从Sep-park C18柱洗脱的血清样品中维生素B1和B2的含量。考察了保留时间、洗脱液体积和比例对样品回收率的影响。
回收率在82.67%至96.89%之间。变异系数<10%。线性相关系数分别为0.9985和0.9995。肥胖人群血清VB1和VB2含量低于健康对照组。
建立了测定血清VB1和VB2的高效液相色谱法。肥胖人群血清VB1和VB2水平较低。
