用于水相铜离子的紫外-可见光可逆但荧光不可逆化学传感器及其在活细胞中的应用。

Ultraviolet-visible light (UV-Vis)-reversible but fluorescence-irreversible chemosensor for copper in water and its application in living cells.

机构信息

Research Institute of Applied Cheemistry, Shanxi University, Taiyuan 030006, People's Republic of China.

出版信息

Anal Chem. 2012 Mar 6;84(5):2219-23. doi: 10.1021/ac202734m. Epub 2012 Feb 17.

DOI:10.1021/ac202734m

Abstract

An ultraviolet-visible light (UV-Vis)-reversible but fluorescence-irreversible chemosensor was developed for the detection of copper. Coordination between the probe, 2-pyridylaldehyde fluorescein hydrazone (FHP), and Cu(2+) gave a reversible UV-Vis response, Storage of the probe-Cu complex resulted in hydrolytic cleavage of the N═C bond, which released the fluorophore (ring-opened fluorescein hydrazine) and gave irreversible fluorescence. Thus, FHP becomes a multifunctional chemosensor, and its reversibility can be controlled by the reaction time. Cu(2+) in living cells could be detected using FHP and general fluorescence methods.

摘要

开发了一种对铜具有紫外可见光（UV-Vis）可逆但荧光不可逆响应的化学传感器。探针 2-吡啶醛荧光素腙（FHP）与 Cu(2+) 的配位作用给出了一个可逆的 UV-Vis 响应，探针-Cu 配合物的储存导致 N═C 键的水解断裂，释放出荧光团（开环荧光素腙）并产生不可逆的荧光。因此，FHP 成为一种多功能化学传感器，其可逆性可以通过反应时间来控制。FHP 可以与常规荧光方法一起用于检测活细胞中的 Cu(2+)。