Sakato N, Rugdech P, Yoda T, Ota A, Zhao Y, Semma M, Suzuki Y, Fujio H
Department of Immunochemistry, Osaka University, Japan.
Immunology. 1990 Oct;71(2):153-7.
An idiotype (Id)-specific long-term cultured T-cell line has been generated from BALB/c mice immunized with M315 (alpha, lambda 2). The cell line comprises both CD3+ and CD4+ but CD8- cells. The T-cell line is stimulated in a class II major histocompatibility complex (MHC)-restricted manner, and is capable of producing interleukin-2 (IL-2) in response to the Id along with Iad-bearing antigen-presenting cells (APC). Fine Id specificity analysis has shown that changes in amino acid residues, Phe-94, Arg-95 and Asn-96, located on the VL-315, resulting from a somatic mutation mechanism of the mouse V lambda 2 gene, contribute to the T-cell activation. Pretreatment of APC with either glutaraldehyde or paraformaldehyde prevented both Fv-315 and VL-315 from triggering the T cells. This suggested that further processing of VL is required for T-cell activation. To clarify this point, we have generated a synthetic peptide, designated P18, which spans residues 91-108 of VL-315. In sharp contrast to VL, prefixed APC were capable of presenting P18 to stimulate the T-cell line to induce IL-2.
用M315（α，λ2）免疫BALB/c小鼠后，已产生一种独特型（Id）特异性长期培养的T细胞系。该细胞系包含CD3 +和CD4 +但CD8 -的细胞。该T细胞系以II类主要组织相容性复合体（MHC）限制的方式受到刺激，并且能够在Id与携带Iad的抗原呈递细胞（APC）共同作用下产生白细胞介素-2（IL-2）。精细的Id特异性分析表明，小鼠Vλ2基因的体细胞突变机制导致位于VL - 315上的氨基酸残基Phe - 94、Arg - 95和Asn - 96发生变化，这有助于T细胞活化。用戊二醛或多聚甲醛预处理APC可阻止Fv - 315和VL - 315触发T细胞。这表明T细胞活化需要VL的进一步加工。为阐明这一点，我们合成了一种名为P18的合成肽，它跨越VL - 315的91 - 108位残基。与VL形成鲜明对比的是，预先处理的APC能够呈递P18以刺激T细胞系诱导IL - 2。
