胎膜早破早产女性羊水可溶性Toll样受体1家族成员
Soluble Toll-like receptor 1 family members in the amniotic fluid of women with preterm prelabor rupture of the membranes.
作者信息
Kacerovsky Marian, Andrys Ctirad, Drahosova Marcela, Musilova Ivana, Hornychova Helena, Lesko Daniel, Tosner Jindrich, Jacobsson Bo
机构信息
Department of Obstetrics and Gynecology, Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
出版信息
J Matern Fetal Neonatal Med. 2012 Sep;25(9):1699-704. doi: 10.3109/14767058.2012.658463. Epub 2012 Mar 5.
OBJECTIVE
To determine soluble Toll-like receptor (sTLR) 1, sTLR2 and sTLR6 concentrations in amniotic fluid (AF) of women with preterm prelabor rupture of membranes (PPROM) and if there is an association with microbial invasion of the amniotic cavity and histological chorioamnionitis (HCA).
METHODS
Cross-sectional study was performed. Forty-two women with singleton PPROM pregnancies at a gestational age between 24 + 0 and 36 + 6 weeks were included in the study (twenty-two women with presence of both microbial invasion of the amniotic cavity and HCA, and 20 women without microbial invasion of the amniotic cavity and HCA). Amniocenteses were performed, and the concentrations of sTLRs were determined by sandwich enzyme-linked immunosorbent assays.
RESULTS
Women with microbial invasions of the amniotic cavity and HCA (n = 22) had significantly higher median sTLR1, sTLR2 and sTLR6 levels than those without (n = 20). (20.4 ng/mL vs. 0.44 ng/mL; p < 0.0001, 577.6 ng/mL vs. 60.7 ng/mL; p < 0.0001 and 0.44 ng/mL vs. 0.26 ng/mL; p = 0.02, respectively).
CONCLUSIONS
Women with microbial invasion of the amniotic cavity and HCA had higher AF sTLR1, 2 and 6 levels.
目的
测定胎膜早破（PPROM）孕妇羊水（AF）中可溶性Toll样受体（sTLR）1、sTLR2和sTLR6的浓度，以及其与羊膜腔微生物入侵和组织学绒毛膜羊膜炎（HCA）之间是否存在关联。
方法
进行横断面研究。纳入42例单胎PPROM妊娠孕妇，孕周在24 + 0至36 + 6周之间（22例存在羊膜腔微生物入侵和HCA，20例无羊膜腔微生物入侵和HCA）。进行羊膜腔穿刺，并通过夹心酶联免疫吸附测定法测定sTLRs的浓度。
结果
存在羊膜腔微生物入侵和HCA的孕妇（n = 22）的sTLR1、sTLR2和sTLR6中位数水平显著高于无上述情况的孕妇（n = 20）。（分别为20.4 ng/mL对0.44 ng/mL；p < 0.0001，577.6 ng/mL对60.7 ng/mL；p < 0.0001，0.44 ng/mL对0.26 ng/mL；p = 0.02）。
结论
存在羊膜腔微生物入侵和HCA的孕妇羊水sTLR1、2和6水平较高。
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