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多发性硬化症和青少年类风湿性关节炎中HLA-DPB1*02和-DPB1*04的DNA分型

DNA typing for HLA-DPB1*02 and -DPB1*04 in multiple sclerosis and juvenile rheumatoid arthritis.

作者信息

Fugger L, Ryder L P, Morling N, Odum N, Friis J, Pedersen F K, Heilmann C, Sandberg-Wollheim M, Svejgaard A

机构信息

Department of Clinical Immunology, State University Hospital, Copenhagen, Denmark.

出版信息

Immunogenetics. 1990;32(3):150-6. doi: 10.1007/BF02114968.

Abstract

DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes (SSOP) has recently been reported. The amplification step may be specific for the HLA-DPB locus, or it may be specific for one or a group of HLA-DPB alleles, thus increasing the discriminatory power of the system. We report the combined use of group-specific DNA in vitro amplification followed by SSOP in typing for DPB102 and DPB104 variants. The method was used to type for these variants in 96 randomly selected, healthy Danes, in 37 patients with pauciarticular juvenile rheumatoid arthritis (PJRA), and in 38 patients with multiple sclerosis (MS). Increased frequencies of the cellularly defined HLA-DPw2 in PJRA and of HLA-DPw4 in MS have previously been reported. In the patient groups, the frequencies of the DPB102 and DPB104 variants did not differ significantly from those expected based on the cellularly defined HLA-DP types of the patients and the frequencies of the DPB102 and DPB104 variants among healthy Danes.

摘要

最近有报道称,利用体外DNA扩增结合序列特异性寡核苷酸探针(SSOP)进行DP基因分型。扩增步骤可能对HLA-DPB基因座具有特异性,也可能对一个或一组HLA-DPB等位基因具有特异性,从而提高了该系统的鉴别能力。我们报告了在对DPB102和DPB104变体进行分型时,联合使用组特异性DNA体外扩增,随后进行SSOP分析。该方法用于对96名随机选择的健康丹麦人、37名少关节型幼年类风湿性关节炎(PJRA)患者和38名多发性硬化症(MS)患者的这些变体进行分型。此前有报道称,PJRA患者中细胞定义的HLA-DPw2频率增加,MS患者中HLA-DPw4频率增加。在患者组中,DPB102和DPB104变体的频率与基于患者细胞定义的HLA-DP类型以及健康丹麦人中DPB102和DPB104变体的频率所预期的频率没有显著差异。

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