利用等位基因特异性DNA体外扩增和等位基因特异性寡核苷酸探针进行HLA-DPB103和HLA-DPB106分型。检测“新型”DPB1*06变体。

Typing for HLA-DPB103 and HLA-DPB106 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of "new" DPB1*06 variants.

作者信息

Fugger L, Morling N, Ryder L P, Odum N, Georgsen J, Svejgaard A

机构信息

Department of Clinical Immunology, State University Hospital (Rigshospitalet), Copenhagen, Denmark.

出版信息

Immunogenetics. 1989;30(3):208-13. doi: 10.1007/BF02421208.

DOI:10.1007/BF02421208

PMID:2777339

Abstract

DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes has recently been reported. The resulting DNA amplification was specific for the HLA-DPB locus. Typing for the individual DPB alleles was exclusively dependent on the hybridizations of the probes but hampered by close sequence homology between different DP alleles yielding complex patterns of reactivity with a panel of probes. We report the combined use of allele-specific DNA in vitro amplification and allele-specific oligonucleotides in typing for DPB103 and DPB106. Complete concordance with PLT typing was observed for the DPB103 alleles, while in the DPB106 group, at least three variant DPB1*06 alleles were identified which have not been described previously.

摘要

最近有报道称，利用体外DNA扩增结合序列特异性寡核苷酸探针进行DP基因分型。所产生的DNA扩增对HLA - DPB基因座具有特异性。个体DPB等位基因的分型完全依赖于探针的杂交，但由于不同DP等位基因之间的序列同源性相近，导致与一组探针产生复杂的反应模式，从而阻碍了分型。我们报告了等位基因特异性DNA体外扩增与等位基因特异性寡核苷酸在DPB103和DPB106分型中的联合应用。对于DPB103等位基因，观察到与血小板分型（PLT分型）完全一致，而在DPB106组中，鉴定出至少三个先前未描述过的DPB1*06变异等位基因。