Departments of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Bochum, Germany.
J Eur Acad Dermatol Venereol. 2012 Feb;26(2):180-5. doi: 10.1111/j.1468-3083.2011.04028.x. Epub 2011 Mar 9.
Injections with phosphatidylcholine- and deoxycholate-containing substances are used to treat localized fat accumulation and lipomas. It is believed that the injected substances induce fat cell destruction with subsequent acute panniculitis followed by a repair process of the treated fat tissue.
We investigated whether necrosis or apoptosis of fat cells was induced by the injected substances.
Samples of fat tissue of lipoma were collected at various times after injection and evaluated by light and electron microscopy, by immunostaining for active caspase-3 and antideoxyribonuclease I, in situ end-labelling (TUNEL staining), and biochemical caspase-3 assays.
Light and electron microscopy showed fat cell necrosis in all areas of the treated lipomas. Low levels of active caspase-3 indicated the absence of apoptosis.
Injection of the lipolytic substances phosphatidylcholine and deoxycholate leads to fat cell necrosis rather than apoptosis. However, additional studies evaluating different dosing and further time points after treatment are necessary.
含有磷脂酰胆碱和脱氧胆酸钠的注射剂被用于治疗局部脂肪堆积和脂肪瘤。据信，注射的物质会引起脂肪细胞破坏，随后发生急性脂肪炎，然后是治疗脂肪组织的修复过程。
我们研究了注射的物质是否会诱导脂肪细胞坏死或凋亡。
在注射后的不同时间采集脂肪瘤的脂肪组织样本，并通过光镜和电镜、针对活性半胱天冬酶-3 的免疫染色、原位末端标记（TUNEL 染色）和生化半胱天冬酶-3 测定进行评估。
光镜和电镜显示治疗性脂肪瘤的所有区域均有脂肪细胞坏死。活性半胱天冬酶-3 的低水平表明不存在细胞凋亡。
注射脂肪分解物质磷脂酰胆碱和脱氧胆酸钠会导致脂肪细胞坏死而不是凋亡。然而，还需要评估不同剂量和治疗后进一步时间点的其他研究。
