Minor T E, Helstrom P B, Nelson D B, D'Alessio D J
J Clin Microbiol. 1979 Apr;9(4):503-6. doi: 10.1128/jcm.9.4.503-506.1979.
A counterimmunoelectrophoresis test was developed for immunoglobulin M (IgM) antibodies to group B coxsackievirus (CB) types 1 through 5. The IgM precipitin line could be identified and differentiated from the IgG line by treating sera with 2-mercaptoethanol. Antigen purity was demonstrated by single precipitin lines occurring only to the homologous antigen when tested with type-specific hyperimmune rabbit sera. Serum pairs from 19 of 22 patients with documented CB type 1,3,4, and 5 infections were positive for IgM antibody to the infecting serotype, whereas 2 of 7 pairs from CB type 2 patients were positive. Heterologous IgM antibodies were present in sera from 14 fo 29 CB patients. Of the 14 patients with heterologous IgM antibodies, 12 also had greater than or equal to 4-fold rises in whole serum neutralizing antibody to heterologous serotypes. Only three control sera from 72 patients with coxsackievirus group A, echovirus, or other viral infections had IgM antibody to CB serotypes.
开发了一种针对B组柯萨奇病毒（CB）1至5型免疫球蛋白M（IgM）抗体的对流免疫电泳试验。通过用2-巯基乙醇处理血清，可识别IgM沉淀线并将其与IgG线区分开来。当用型特异性超免疫兔血清进行检测时，仅对同源抗原出现单一沉淀线，证明了抗原的纯度。在记录有CB 1、3、4和5型感染的22例患者中，19例患者的血清对感染血清型的IgM抗体呈阳性，而7例CB 2型患者血清对中的2例呈阳性。29例CB患者中有14例血清中存在异源IgM抗体。在这14例有异源IgM抗体的患者中，12例对异源血清型的全血清中和抗体也有大于或等于4倍的升高。在72例A组柯萨奇病毒、埃可病毒或其他病毒感染患者中，只有3份对照血清含有针对CB血清型的IgM抗体。
