Paily K P
Vector Control Research Centre, Pondicherry.
Indian J Med Res. 1990 Jul;91:298-302.
An attempt was made to develop an alternative method of mass culturing for R. iyengari instead of the usual sand culture method. Fifty pairs each of post-parasitic juveniles were seeded in moist sandbed and beakers containing tap water and distilled water and examined for exsheathing, egg-laying and egg-hatching. All post-parasitic juveniles in the moist sandbed had moulted by 7th day whereas in tap water and distilled water it lasted up to 11th day. Maximum numbers of eggs were observed on day 14 in sandbed (15/ml), day 16 in tap water (24/ml) and day 29 in distilled water (28/ml). The preparasites obtained from moist sandbed, tap water and distilled water did not exhibit any difference in their infectivity to mosquito larvae. The eggs of the nematode obtained from a culture in distilled water maintained at 30 +/- 2 degrees C for 60 days, when treated with CO2 (18 to 556 ppm) showed enhanced rate of egg hatching (73-98% compared with 11.5% in the untreated ones). CO2 treatment did not affect the infectivity of the preparasites that hatched from the CO2-treated eggs.
人们试图开发一种用于伊氏罗索线虫大规模培养的替代方法，以取代常规的沙培法。将50对寄生后幼虫分别接种在潮湿的沙床以及装有自来水和蒸馏水的烧杯中，观察其脱鞘、产卵和卵孵化情况。潮湿沙床中的所有寄生后幼虫在第7天已蜕皮，而在自来水和蒸馏水中则持续到第11天。在第14天观察到沙床中产卵数量最多（15个/毫升），自来水中在第16天（24个/毫升），蒸馏水中在第29天（28个/毫升）。从潮湿沙床、自来水和蒸馏水中获得的感染前期幼虫对蚊虫幼虫的感染力没有差异。将在30±2℃下于蒸馏水中培养60天的线虫卵用二氧化碳（18至556 ppm）处理后，卵孵化率提高（73 - 98%，未处理的为11.5%）。二氧化碳处理不影响从经二氧化碳处理的卵中孵化出的感染前期幼虫的感染力。
