Department of Obstetrics & Gynecology and Reproduction, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Anim Reprod Sci. 2012 Jan;130(1-2):9-15. doi: 10.1016/j.anireprosci.2011.12.013. Epub 2012 Jan 10.
The objectives were to investigate relationships among concentrations of nitric oxide (NO), estradiol 17 beta (E2), and progesterone (P4) in follicular fluids (FF), and quality of cumulus oocyte complexes (COCs) with respect to perifollicular blood flow (FBF). In Experiment I, follicles (138) were classified according to the presence or absence of FBF (assessed with transvaginal Doppler ultrasonography) and diameter of follicles (small, 2-4 mm; medium, 5-8 mm; and large, ≥9 mm). Concentrations of NO in FF did not differ significantly among these size categories. However, NO concentrations in FF with FBF (54.4 ± 7.4 μmol/l) were higher (P<0.05) than in those without FBF (36.6 ± 4.1 μmol/l). There was a positive correlation (r=0.30, P<0.05) between NO concentrations and the E2:P4 in FF. Rate of E2 active (E2:P4 ≥ 1) follicles were numerically 1.2 (0.8-1.8) times higher in follicles with FBF (38.1%) compared to those without FBF (25.0%). Moreover, rates of E2 active follicles were 6.1 (0.7-55.2) and 1.3 (0.1-17.3) times higher (P<0.06) in large (43.3%) and medium (14.3%) compared to small follicles (11.1%), respectively. In Experiment II, quality of COCs from 2 to 8 mm follicles, obtained by transvaginal ovum pick up (OPU), was investigated with respect to FBF. Odds ratio to obtain higher quality COCs from follicles with FBF (47.1%) was 3.3 (1.1-9.6) fold higher (P<0.05) compared to those from follicles without FBF (14.6%). In conclusion, E2:P4, and NO concentrations in FF, as well as FBF, could be used to determine the functionality of ovarian follicles in cows. Moreover, determination of FBF could be useful to predict quality of COCs in cattle.
目的在于探究卵泡液（FF）中一氧化氮（NO）、雌二醇 17β（E2）和孕酮（P4）的浓度与周围血流（FBF）之间的关系，以及与卵丘-卵母细胞复合体（COCs）质量的关系。在实验 I 中，根据是否存在 FBF（经阴道多普勒超声评估）和卵泡直径（小，2-4mm；中，5-8mm；大，≥9mm）对卵泡（138 个）进行分类。FF 中 NO 的浓度在这些大小类别之间没有显著差异。然而，有 FBF 的 FF 中的 NO 浓度（54.4±7.4μmol/l）高于没有 FBF 的 FF（36.6±4.1μmol/l）（P<0.05）。NO 浓度与 FF 中 E2:P4 呈正相关（r=0.30，P<0.05）。有 FBF 的卵泡（38.1%）中 E2 活性（E2:P4≥1）卵泡的比例比没有 FBF 的卵泡（25.0%）高 1.2（0.8-1.8）倍。此外，大卵泡（43.3%）和中卵泡（14.3%）中 E2 活性卵泡的比例分别比小卵泡（11.1%）高 6.1（0.7-55.2）和 1.3（0.1-17.3）倍（P<0.06）。在实验 II 中，通过经阴道卵母细胞抽吸（OPU）获取 2-8mm 卵泡的 COCs 质量，对其与 FBF 的关系进行了研究。有 FBF 的卵泡获得高质量 COCs 的几率（47.1%）是没有 FBF 的卵泡（14.6%）的 3.3（1.1-9.6）倍（P<0.05）。总之，FF 中 E2:P4、NO 浓度以及 FBF 可以用于确定奶牛卵巢卵泡的功能。此外，确定 FBF 可能有助于预测牛的 COCs 质量。
